anti-Human Complement Component 5 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended Complement Component 5 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Complement Component 5 (C5) Antikörper
  • C5
  • LOC565774
  • C5a
  • C5b
  • CPAMD4
  • Hc
  • RGD1561905
  • He
  • complement component 5
  • similar to complement component C5-1
  • hemolytic complement
  • C5
  • LOC565774
  • Hc
Reaktivität
Human
144
22
10
7
2
2
2
1
Wirt
Maus
85
75
11
6
Klonalität
Monoklonal
Konjugat
Unkonjugiert
14
12
6
6
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
103
82
28
22
18
15
13
10
10
9
8
7
6
5
4
3
2
2
2
2
1
1
1
1
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Produktnummer ABIN112155
341,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN111066 EIA IHC (fro) WB Mouse IgG1 Anmelden zum Anzeigen HCC5b-1 (neo)
1 ABIN568577 EIA ID IHC (fro) IF IP FITC Goat IgG Anmelden zum Anzeigen Polyclonal
1 ABIN113312 ID IHC (fro) Sheep IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1106791 Func EIA IHC (fro) IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 2952 1
1 ABIN1106792 Func EIA IHC (fro) WB Mouse IgG2a Anmelden zum Anzeigen 2942 1
1 ABIN215712 IHC (fro) ELISA WB Mouse IgG1 Anmelden zum Anzeigen HCC5-1
1 ABIN1077949 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 678-751 Anmelden zum Anzeigen Polyclonal
1 ABIN284070 IHC (fro) ELISA Mouse IgG1 Anmelden zum Anzeigen HCC5-1

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General

Antigen Complement Component 5 (C5) Antikörper
Reaktivität Human
(144), (22), (10), (7), (2), (2), (2), (1)
Wirt Maus
(85), (75), (11), (6)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(14), (12), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(103), (82), (28), (22), (18), (15), (13), (10), (10), (9), (8), (7), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Spezifität HCC5.1 allows detection of native complement component C5 and discrimination from activated C5b-9 complexes, especially in different forms of glomerulonephritis. This HCC5.1 antibody reacts with both native C5 as well as with C5b. Native C5 is only detected with HCC5.1, while C5b is detected by both HCC5.1 and HCC5.2. Polypeptide Reacting: Mr 200,000 polypeptide of native complement component C5.Polypeptide Reacting
Kreuzreaktivität (Details) Species reactivity (tested):Human.
Reinigung Affinity Chromatography on Protein A
Immunogen Human complement component C5
Klon HCC5-1
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Complement C5 (C5 Antibody Abstract)
Hintergrund The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b, resulting in activation of the lytic pahway C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis. C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5
Gen-ID 727
NCBI Accession NP_001726
UniProt P01031
Forschungsgebiet Complement Factors
Pathways Komplementsystem, Carbohydrate Homeostasis

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Immunohistochemistry on frozen sections: 1: 10, 1h at RT. Western Blot. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Rekonstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Vorsichtsmaßnahmen Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.