p53 ELISA Kit

Produktnummer ABIN921118

Produktdetails p53 ELISA Kit

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: AA 1-393

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human P53

Marke: PicoKine™

Proben: Cell Lysate

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: M1-D393

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: M1-D393

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NSO, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human P53 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human P53 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1328, Standard deviation: 78.4, CV(%): 5.9
• Sample 2: n=16, Mean(pg/ml): 2789, Standard deviation: 173, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 5434, Standard deviation: 353.21, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 1502, Standard deviation: 103.7, CV(%): 6.9
• Sample 2: n=24, Mean(pg/ml): 2891, Standard deviation: 214, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 6120, Standard deviation: 508, CV(%): 8.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für p53 ELISA Kit (ABIN921118)

Hasanzadeh, Baghban, Mokhtarzadeh, Shadjou, Mahboob: "An innovative immunosensor for detection of tumor suppressor protein p53 in unprocessed human plasma and cancer cell lysates." in: International journal of biological macromolecules, Vol. 105, Issue Pt 1, pp. 1337-1348, (2018) (PubMed).

Lu, Lin, Gu, Li, Guo, Wang, Qiu: "Antitumor effects of crocin on human breast cancer cells." in: International journal of clinical and experimental medicine, Vol. 8, Issue 11, pp. 20316-22, (2016) (PubMed).

Srivastava, Narang, Bhatia, Saluja: "Expression of Heat Shock Protein 70 Gene and Its Correlation with Inflammatory Markers in Essential Hypertension." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151060, (2016) (PubMed).

Sun, Yang, Wang, Sun, Dong: "Crocin attenuates cisplatin-induced liver injury in the mice." in: Human & experimental toxicology, Vol. 33, Issue 8, pp. 855-62, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Li, Chu, Gao, Li: "Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 80-9, (2009) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: TP53 (TP53 Produkte)

Synonyme: BCC7 ELISA Kit, LFS1 ELISA Kit, P53 ELISA Kit, TRP53 ELISA Kit, p53 ELISA Kit, Trp53 ELISA Kit, brp53 ELISA Kit, drp53 ELISA Kit, etID22686.5 ELISA Kit, fb40d06 ELISA Kit, wu:fb40d06 ELISA Kit, zgc:111919 ELISA Kit, Tp53 ELISA Kit, bbl ELISA Kit, bfy ELISA Kit, bhy ELISA Kit, p44 ELISA Kit, tp53 ELISA Kit, Xp53 ELISA Kit, TP53 ELISA Kit, tumor protein p53 ELISA Kit, cellular tumor antigen p53 ELISA Kit, transformation related protein 53 ELISA Kit, tumor protein p53 L homeolog ELISA Kit, TP53 ELISA Kit, CpipJ_CPIJ002758 ELISA Kit, Tp53 ELISA Kit, tp53 ELISA Kit, Trp53 ELISA Kit, tp53.L ELISA Kit

Hintergrund:

Protein Function: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

Background: P53(also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton( kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Synonyms: Cellular tumor antigen p53 ,TP53 ,

Full Gene Name: Cellular tumor antigen p53

Cellular Localisation: Cytoplasm. Nucleus.

Gen-ID: 7157

UniProt: K7PPA8

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt