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IL1A ELISA Kit

IL1A Reaktivität: Maus AA 115-270 Colorimetric Sandwich ELISA 4.7-300 pg/mL Cell Culture Supernatant, Serum
Produktnummer ABIN921060
  • Target Alle IL1A ELISA Kits anzeigen
    IL1A (Interleukin 1 alpha (IL1A))
    Bindungsspezifität
    AA 115-270
    Reaktivität
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    4.7-300 pg/mL
    Untere Nachweisgrenze
    4.7 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 alpha
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli,S115-S270
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <1pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: S115-S270
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    mouse IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 alpha amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL mouse IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-1 alpha standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 37, Standard deviation: 1.924, CV(%): 5.2
    • Sample 2: n=16, Mean(pg/ml): 89, Standard deviation: 3.83, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 187, Standard deviation: 7.3, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 46, Standard deviation: 2.99, CV(%): 6.5
    • Sample 2: n=24, Mean(pg/ml): 98, Standard deviation: 5.3, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 175, Standard deviation: 10.85, CV(%): 6.2
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).

    Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

    Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

    Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).

    Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).

    Kouadir, Yang, Tan, Shi, Lu, Zhang, Yin, Zhou, Zhao: "CD36 participates in PrP(106-126)-induced activation of microglia." in: PLoS ONE, Vol. 7, Issue 1, pp. e30756, (2012) (PubMed).

  • Target Alle IL1A ELISA Kits anzeigen
    IL1A (Interleukin 1 alpha (IL1A))
    Andere Bezeichnung
    IL1A (IL1A Produkte)
    Synonyme
    IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
    Hintergrund

    Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

    Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18 kDa.

    Synonyms: Interleukin-1 alpha,IL-1 alpha,Il1a,

    Full Gene Name: Interleukin-1 alpha

    Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
    Gen-ID
    16175
    UniProt
    P01582
    Pathways
    NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints
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