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ALT ELISA Kit

ALT Reaktivität: Maus Colorimetric Sandwich ELISA 0.156-10 mU/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Produktnummer ABIN832245
  • Target Alle ALT ELISA Kits anzeigen
    ALT (Alanine Aminotransferase (ALT))
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156-10 mU/mL
    Untere Nachweisgrenze
    0.156 mU/mL
    Applikation
    ELISA
    Verwendungszweck
    For the quantitative determination of mouse alanine aminotransferase (ALT/GPT) concentrations in serum, plasma, cell culture supernates, cell lysates.
    Proben
    Serum, Plasma, Cell Culture Supernatant, Cell Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of mouse ALT.
    Kreuzreaktivität (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivität
    0.039 mU/mL
    Bestandteile
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
    Featured
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  • Applikationshinweise
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Kommentare

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALT bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Aufbereitung der Reagenzien
    • Biotin-antibody (1×) - Centrifuge the vial before opening.
      Biotin-antibody requires a 100-fold dilution. The suggested dilution is 10µL of Biotin-antibody + 990µL of Biotin-antibody Diluent.
    • HRP-avidin (1×) - Centrifuge the vial before opening.
      HRP-avidin requires a 100-fold dilution. The suggested dilution is 10µL of HRP-avidin + 990µL of HRP-avidin Diluent.
    • Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20mL of Wash Buffer Concentrate (25×) into deionized or distilled water to prepare 500mL of Wash Buffer (1×).
    • Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
      Reconstitute the Standard with 1ml of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
      Pipette 250µL of Sample Diluent into each tube. Use the stock solution to produce a 2-fold dilution series. Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard (0ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25°C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction. Avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL when pipetting.
    • It is recommended to use distilled water to prepare reagents and samples. Using contaminated water or container for reagent preparation will influence detection result.
    Testpräzision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handhabung
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Haltbarkeit
    6 months
  • Xiahou, Wang, Shen, Zhu, Xu, Hu, Guo, Li, Tian, Liu, Liang: "NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury." in: Nature communications, Vol. 8, Issue 1, pp. 950, (2018) (PubMed).

    Li, Lu, Liu, Liang, Wang, Wang, Li, Yao, Zhang, Wen, Zhang, Li, Lv, He, Guo, Guan, Yu, Dang, Wu, Li, Chen, Jiang, Sun, Zhang, Lu, Zhang: "A water-soluble nucleolin aptamer-paclitaxel conjugate for tumor-specific targeting in ovarian cancer." in: Nature communications, Vol. 8, Issue 1, pp. 1390, (2018) (PubMed).

    Chen, Zhou, Wu, Wang, Wang: "FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice." in: Journal of physiology and biochemistry, (2015) (PubMed).

    Krzystolik, Dziedziejko, Safranow, Kurzawski, Ra?, Sagasz-Tysiewicz, Poncyljusz, Jakubowska, Chlubek, Ra?: "Is plasma soluble CD36 associated with cardiovascular risk factors in early onset coronary artery disease patients?" in: Scandinavian journal of clinical and laboratory investigation, Vol. 75, Issue 5, pp. 398-406, (2015) (PubMed).

  • Target Alle ALT ELISA Kits anzeigen
    ALT (Alanine Aminotransferase (ALT))
    Andere Bezeichnung
    Alanine Aminotransferase (ALT/GPT) (ALT Produkte)
    Synonyme
    AAT1 ELISA Kit, ALT1 ELISA Kit, GPT1 ELISA Kit, GPT ELISA Kit, Gpt1 ELISA Kit, An11g02620 ELISA Kit, AO090003000164 ELISA Kit, 233.t00009 ELISA Kit, 24.t00016 ELISA Kit, 1300007J06Rik ELISA Kit, 2310022B03Rik ELISA Kit, ALT ELISA Kit, Gpt-1 ELISA Kit, AU021132 ELISA Kit, Dpagt2 ELISA Kit, Gnpta ELISA Kit, gpt ELISA Kit, ALANINE AMINOTRANSFERAS ELISA Kit, AtAlaAT1 ELISA Kit, AtAlaATc ELISA Kit, T13M22.3 ELISA Kit, T13M22_3 ELISA Kit, alanine aminotransferas ELISA Kit, glutamic--pyruvic transaminase ELISA Kit, glutamic-pyruvate transaminase (alanine aminotransferase) ELISA Kit, alanine aminotransferase ELISA Kit, alanine aminotransferase 1 ELISA Kit, glutamic pyruvic transaminase, soluble ELISA Kit, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA Kit, alanine aminotransferase 2-like ELISA Kit, Alanine aminotransferase 1, mitochondrial ELISA Kit, GPT ELISA Kit, Gpt ELISA Kit, Tb927.1.3950 ELISA Kit, ANI_1_368094 ELISA Kit, AOR_1_284154 ELISA Kit, ALAT_1 ELISA Kit, ALAAT1 ELISA Kit, EHI_159710 ELISA Kit, EHI_096750 ELISA Kit, LOC5572541 ELISA Kit, ALAT ELISA Kit, AAT2 ELISA Kit, Dpagt1 ELISA Kit, LOC100537633 ELISA Kit, AlaAT1 ELISA Kit
    Hintergrund
    Synonyms: AAT1, ALT1, GPT1, glutamic pyruvate transaminase|glutamic-alanine transaminase 1
    UniProt
    Q8QZR5
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