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IL-8 ELISA Kit

IL8 Reaktivität: Human Colorimetric Sandwich ELISA 1-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN625029
  • Target Alle IL-8 (IL8) ELISA Kits anzeigen
    IL-8 (IL8) (Interleukin 8 (IL8))
    Reaktivität
    • 14
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1-600 pg/mL
    Untere Nachweisgrenze
    1 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivität
    < 1 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Aufbereitung der Reagenzien
    1. Bring all reagents and samples to room temperature (18-25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernates and urine. Suggested dilution for normal serum/plasma: 2 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 800 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine, Assay Diluent B should be diluted 5-fold with deionized or distilled water before use) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 10 µL IL-8 standard from the vial of Item C, into a tube with 823.3 µL Assay Diluent A or 1x Assay Diluent B to prepare a 600 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200myl 200 µL 200 µL 200 µL 200 µL 10 µL standard + 823.3 µL 600 200 66.7 22.2 7.4 2.5 0.8 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 600-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 25 µL of HRP-Streptavidin concentrate into a tube with 15 ml 1x Assay Diluent B to prepare a final 600 fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    Testdurchführung
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    Ergebnisberechnung

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay Human IL-8 concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent A Human IL-8 concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B
    Sensitivity: The minimum detectable dose of IL-8 is typically less than 1 pg/mL.
    Recovery: Recovery was determined by spiking various levels of recombinant human IL-8 into normal human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 95.34 84-107 Plasma 93.85 83-104 Cell culture media 96.18 85-106
    Linearity: Sample Type Serum Plasma Cell culture media 1:2 Average % of Expected 96 94 98 Range ( %) 85-104 83-105 85-107 1:4 Average % of Expected 95 92 96 Range ( %) 84-106 82-104 84-105
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    Testpräzision
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Scuruchi, DAscola, Avenoso, Zappone, Mandraffino, Campo, Campo: "miR9 inhibits 6-mer HA-induced cytokine production and apoptosis in human chondrocytes by reducing NF-kB activation." in: Archives of biochemistry and biophysics, Vol. 718, pp. 109139, (2022) (PubMed).

    Duckworth, Zhang, Carroll, Ethier, Cheung: "Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression." in: Oncogene, Vol. 35, Issue 31, pp. 4036-47, (2017) (PubMed).

    Xu, Li, Huang, Lin, Gai, Chen: "Impact of IL-8-251A/T gene polymorphism on severity of disease caused by enterovirus 71 infection." in: Archives of virology, Vol. 161, Issue 1, pp. 203-7, (2016) (PubMed).

    Spaks, Jaunalksne, Spaka, Chudasama, Pirtnieks, Krievins: "Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer." in: Anticancer research, Vol. 35, Issue 12, pp. 6979-83, (2016) (PubMed).

    Li, Lin, Yu, Zhang, Xu, Hu, Liu, Wang, Nie, Sun, Gai, Chen: "Association of Enterovirus 71 encephalitis with the interleukin-8 gene region in Chinese children." in: Infectious diseases (London, England), Vol. 47, Issue 6, pp. 418-22, (2016) (PubMed).

    Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

    Perrigue, Silva, Warden, Feng, Reid, Mota, Joseph, Tian, Glackin, Gutova, Najbauer, Aboody, Barish: "The Histone Demethylase Jumonji Coordinates Cellular Senescence Including Secretion of Neural Stem Cell-attracting Cytokines." in: Molecular cancer research : MCR, (2015) (PubMed).

    Kuntz, Asseburg, Dold, Römpp, Fröhling, Kunz, Rudloff: "Inhibition of low-grade inflammation by anthocyanins from grape extract in an in vitro epithelial-endothelial co-culture model." in: Food & function, Vol. 6, Issue 4, pp. 1136-49, (2015) (PubMed).

    Ikeno, Apel, Zouboulis, Luger, Böhm: "L-Ascorbyl-2-phosphate attenuates NF-?B signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion." in: Archives of dermatological research, Vol. 307, Issue 7, pp. 595-605, (2015) (PubMed).

    Zhou, Gu, Gu, He, Bi, Chen, Li et al.: "Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation ..." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 6, pp. 2383-401, (2015) (PubMed).

    Huggins, Pearce, Peri, Neumann, Cockerill, Pirianov: "A novel small molecule TLR4 antagonist (IAXO-102) negatively regulates non-hematopoietic toll like receptor 4 signalling and inhibits aortic aneurysms development." in: Atherosclerosis, Vol. 242, Issue 2, pp. 563-70, (2015) (PubMed).

    Rabi, Todorović-Raković, Vujasinović, Milovanović, Nikolić-Vukosavljević: "Markers of progression and invasion in short term follow up of untreated breast cancer patients." in: Cancer biomarkers : section A of Disease markers, Vol. 15, Issue 6, pp. 745-54, (2015) (PubMed).

    Patra, Ghosh, Modak, Bandopadhyay, Saha, Mukhopadhyay: "Status of circulating immune complexes, IL8 titers and cryoglobulins in patients with dengue infection." in: Indian journal of experimental biology, Vol. 53, Issue 11, pp. 719-25, (2015) (PubMed).

    Ye, Xu, Lou, Jin, Miao, Ye, Xi: "Anti-inflammatory effects of hinokitiol on human corneal epithelial cells: an in vitro study." in: Eye (London, England), Vol. 29, Issue 7, pp. 964-71, (2015) (PubMed).

    Gao, Guan, Li, Su, Wang: "Ameliorating effects of low tidal volume ventilation with associated hypercapnia on pneumoperitoneum-induced lung injury by inhibition of Toll-like receptor 4." in: International journal of clinical and experimental medicine, Vol. 8, Issue 2, pp. 1814-23, (2015) (PubMed).

    Sun, Wang, Li, Zhang, Jin, Chen, Pang, Yu, He, Liu, Liu: "Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia/Reperfusion Injury in Mice." in: BioMed research international, Vol. 2015, pp. 395895, (2015) (PubMed).

    Nandi, Bishayi et al.: "Host antioxidant enzymes and TLR-2 neutralization modulate intracellular survival of Staphylococcus aureus: Evidence of the effect of redox balance on host pathogen relationship during acute ..." in: Microbial pathogenesis, Vol. 89, pp. 114-27, (2015) (PubMed).

    Reyes-Quiroz, Alba, Santa-María, Saenz, Geniz, Jiménez, Ramírez, Martín-Nieto, Pintado, Sobrino: "Platelet-activating factor downregulates the expression of liver X receptor-? and its target genes in human neutrophils." in: The FEBS journal, Vol. 281, Issue 3, pp. 970-82, (2014) (PubMed).

    Capó, Martorell, Llompart, Sureda, Tur, Pons: "Docosahexanoic acid diet supplementation attenuates the peripheral mononuclear cell inflammatory response to exercise following LPS activation." in: Cytokine, Vol. 69, Issue 2, pp. 155-64, (2014) (PubMed).

    Miethling-Graff, Rumpker, Richter, Verano-Braga, Kjeldsen, Brewer, Hoyland, Rubahn, Erdmann: "Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 28, Issue 7, pp. 1280-9, (2014) (PubMed).

  • Target Alle IL-8 (IL8) ELISA Kits anzeigen
    IL-8 (IL8) (Interleukin 8 (IL8))
    Andere Bezeichnung
    IL-8 / CXCL8 (IL8 Produkte)
    Synonyme
    CXCL8 ELISA Kit, GCP-1 ELISA Kit, GCP1 ELISA Kit, LECT ELISA Kit, LUCT ELISA Kit, LYNAP ELISA Kit, MDNCF ELISA Kit, MONAP ELISA Kit, NAF ELISA Kit, NAP-1 ELISA Kit, NAP1 ELISA Kit, IL-8 ELISA Kit, AMCF-I ELISA Kit, il8 ELISA Kit, IL8 ELISA Kit, si:dkey-151b16.2 ELISA Kit, cxcli2 ELISA Kit, cxcl8 ELISA Kit, gcp-1 ELISA Kit, il-8 ELISA Kit, lynap ELISA Kit, mdncf ELISA Kit, monap ELISA Kit, nap-1 ELISA Kit, C-X-C motif chemokine ligand 8 ELISA Kit, interleukin 8 ELISA Kit, chemokine (C-X-C motif) ligand 8a ELISA Kit, chemokine (C-X-C motif) ligand 1-like ELISA Kit, chemokine (C-X-C motif) ligand 8 L homeolog ELISA Kit, CXCL8 ELISA Kit, il8 ELISA Kit, cxcl8a ELISA Kit, IL8 ELISA Kit, Cxcl8 ELISA Kit, LOC422654 ELISA Kit, cxcl8.L ELISA Kit
    Hintergrund
    Interleukin-8 (IL-8) is a member of a family of structurally-related low molecular weight proinflammatory factors known as chemokines. IL-8 is produced by stimulated monocytes but not by tissue macrophages and T-lymphocytes. IL-8 is a non-glycosylated protein of 8 kDa (72 amino acids). It is produced by processing of a precursor protein of 99 amino acids. The Human IL-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-8 in serum, plasma, cell culture supernates and urine. This assay employs an antibody specific for human IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    Gen-ID
    3576
    UniProt
    P10145
    Pathways
    TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie
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