VWF ELISA Kit

Produktnummer ABIN5659978

Produktdetails VWF ELISA Kit

Target: VWF (Von Willebrand Factor (VWF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 12.35 ng/mL - 1000 ng/mL

Untere Nachweisgrenze: 12.35 ng/mL

Applikation: ELISA

Proben: Plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF). No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed.

Sensitivität: 4.97 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: Von Willebrand Factor (VWF Produkte)

Synonyme: VWF ELISA Kit, si:ch1073-474e24.1 ELISA Kit, F8VWF ELISA Kit, VWD ELISA Kit, 6820430P06Rik ELISA Kit, AI551257 ELISA Kit, B130011O06Rik ELISA Kit, C630030D09 ELISA Kit, von Willebrand factor ELISA Kit, Von Willebrand factor ELISA Kit, VWF ELISA Kit, vwf ELISA Kit, Vwf ELISA Kit

Hintergrund:

Gene Name: Von Willebrand Factor

Gene Aliases: F8VWF, VWD, von Willebrand antigen 2

Gen-ID: 7450

UniProt: P04275