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Thymopoietin ELISA Kit

TMPO Reaktivität: Human Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5659214
  • Target Alle Thymopoietin (TMPO) ELISA Kits anzeigen
    Thymopoietin (TMPO)
    Reaktivität
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    7.81 pg/mL - 500 pg/mL
    Untere Nachweisgrenze
    7.81 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Thymopoietin (TMPO). No significant cross-reactivity or interference between Thymopoietin (TMPO) and analogues was observed.
    Sensitivität
    12.9 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymopoietin (TMPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymopoietin (TMPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymopoietin (TMPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymopoietin (TMPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Thymopoietin (TMPO) ELISA Kits anzeigen
    Thymopoietin (TMPO)
    Andere Bezeichnung
    Thymopoietin (TMPO Produkte)
    Synonyme
    LAP2 ELISA Kit, CMD1T ELISA Kit, LEMD4 ELISA Kit, PRO0868 ELISA Kit, TP ELISA Kit, lap2 ELISA Kit, LAP2beta ELISA Kit, 5630400D24Rik ELISA Kit, AI195756 ELISA Kit, AI606875 ELISA Kit, AW214352 ELISA Kit, AW547477 ELISA Kit, tmpo ELISA Kit, wu:fc26f12 ELISA Kit, wu:fd21f02 ELISA Kit, wu:fi29h05 ELISA Kit, thymopoietin ELISA Kit, thymopoietin S homeolog ELISA Kit, thymopoietin a ELISA Kit, TMPO ELISA Kit, Tmpo ELISA Kit, tmpo.S ELISA Kit, tmpoa ELISA Kit
    Hintergrund

    Gene Name: Thymopoietin

    Gene Aliases: TP, LAP2, CMD1T, LEMD4, TP5, Splenin, Lamina-Associated Polypeptide 2,Isoforms Beta/Gamma, LEM Domain Containing 4, Thymopoietin-related peptide isoforms beta/gamma

    Gen-ID
    7112
    UniProt
    P42167
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