Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

SRY ELISA Kit

SRY Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658764
  • Target Alle SRY ELISA Kits anzeigen
    SRY (Sex Determining Region Y (SRY))
    Reaktivität
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Sex Determining Region Y (SRY). No significant cross-reactivity or interference between Sex Determining Region Y (SRY) and analogues was observed.
    Sensitivität
    0.055 ng/mL
    Featured
    Zu unserem meistverkauften SRY ELISA Kit
    Top Product
    Discover our top product SRY ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Determining Region Y (SRY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Determining Region Y (SRY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Determining Region Y (SRY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Determining Region Y (SRY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Determining Region Y (SRY) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Determining Region Y (SRY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle SRY ELISA Kits anzeigen
    SRY (Sex Determining Region Y (SRY))
    Andere Bezeichnung
    Sex Determining Region Y (SRY Produkte)
    Synonyme
    SRXX1 ELISA Kit, SRXY1 ELISA Kit, TDF ELISA Kit, TDY ELISA Kit, SRYGENE ELISA Kit, Sry1 ELISA Kit, Sry3BI ELISA Kit, Tdf ELISA Kit, Tdy ELISA Kit, SRY ELISA Kit, sex determining region Y ELISA Kit, sex determining region of Chr Y ELISA Kit, SRY ELISA Kit, Sry ELISA Kit
    Hintergrund

    Gene Name: Sex Determining Region Y

    Gene Aliases: TDF, TDY, Testis-Determining Factor

    Gen-ID
    6736
    UniProt
    Q05066
Sie sind hier:
Kundenservice