PTP4A3 ELISA Kit

Produktnummer ABIN5658260

Produktdetails PTP4A3 ELISA Kit

Target: PTP4A3 (Protein Tyrosine Phosphatase Type IVA, Member 3 (PTP4A3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) and analogues was observed.

Sensitivität: 0.118 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PTP4A3

Target: PTP4A3 (Protein Tyrosine Phosphatase Type IVA, Member 3 (PTP4A3))

Andere Bezeichnung: Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3 Produkte)

Synonyme: PRL-3 ELISA Kit, PRL-R ELISA Kit, PRL3 ELISA Kit, wu:fc54b05 ELISA Kit, wu:fv52d11 ELISA Kit, zgc:77109 ELISA Kit, prl-3 ELISA Kit, prl3 ELISA Kit, ptpcaax3 ELISA Kit, PTP4A3 ELISA Kit, AV088979 ELISA Kit, Prl-3 ELISA Kit, pPtp4a3 ELISA Kit, protein tyrosine phosphatase type IVA, member 3 ELISA Kit, protein tyrosine phosphatase type IVA, member 3 L homeolog ELISA Kit, protein tyrosine phosphatase 4a3 ELISA Kit, PTP4A3 ELISA Kit, ptp4a3 ELISA Kit, ptp4a3.L ELISA Kit, Ptp4a3 ELISA Kit

Hintergrund:

Gene Name: Protein Tyrosine Phosphatase Type IVA 3

Gene Aliases: PRL3, PRL-3, PRL-R, Protein-tyrosine phosphatase of regenerating liver 3, Protein-tyrosine phosphatase 4a3

Gen-ID: 11156

UniProt: O75365