PTGDS ELISA Kit

Produktnummer ABIN5658139

Produktdetails PTGDS ELISA Kit

Target: PTGDS (Prostaglandin D2 Synthase (PTGDS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Prostaglandin D2 Synthase, Hematopoietic (PTGDS). No significant cross-reactivity or interference between Prostaglandin D2 Synthase, Hematopoietic (PTGDS) and analogues was observed.

Sensitivität: 34 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin D2 Synthase, Hematopoietic (PTGDS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin D2 Synthase, Hematopoietic (PTGDS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin D2 Synthase, Hematopoietic (PTGDS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin D2 Synthase, Hematopoietic (PTGDS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin D2 Synthase, Hematopoietic (PTGDS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin D2 Synthase, Hematopoietic (PTGDS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PTGDS

Target: PTGDS (Prostaglandin D2 Synthase (PTGDS))

Andere Bezeichnung: Prostaglandin D2 Synthase, Hematopoietic (PTGDS Produkte)

Synonyme: 6PGD ELISA Kit, GSTS ELISA Kit, GSTS1-1 ELISA Kit, PGD2 ELISA Kit, PGDS ELISA Kit, L-PGDS ELISA Kit, LPGDS ELISA Kit, PDS ELISA Kit, PGDS2 ELISA Kit, H-PGDS ELISA Kit, Ptgds2 ELISA Kit, 21kDa ELISA Kit, Ptgs3 ELISA Kit, PH2DISO ELISA Kit, phosphogluconate dehydrogenase ELISA Kit, hematopoietic prostaglandin D synthase ELISA Kit, prostaglandin D2 synthase ELISA Kit, prostaglandin D2 synthase (brain) ELISA Kit, prostaglandin D synthase ELISA Kit, prostaglandin D2 synthase 21kDa (brain) ELISA Kit, PGD ELISA Kit, HPGDS ELISA Kit, PTGDS ELISA Kit, Hpgds ELISA Kit, Ptgds ELISA Kit, LOC100009453 ELISA Kit

Hintergrund:

Gene Name: Prostaglandin D2 Synthase, Hematopoietic

Gene Aliases: HPGDS, GSTS, H-PGDS, PDS, PGD2, PGDS, PGDS2, Glutathione S-Transferase Sigma, Glutathione-requiring prostaglandin D synthase, Prostaglandin-H2 D-isomerase

Gen-ID: 27306

UniProt: O60760