Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MMP10 ELISA Kit

MMP10 Reaktivität: Maus Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
Produktnummer ABIN5656700
  • Target Alle MMP10 ELISA Kits anzeigen
    MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))
    Reaktivität
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    6.25 pg/mL - 400 pg/mL
    Untere Nachweisgrenze
    6.25 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10). No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.
    Sensitivität
    2.35 pg/mL
    Featured
    Zu unserem meistverkauften MMP10 ELISA Kit
    Top Product
    Discover our top product MMP10 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 10 (MMP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 10 (MMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 10 (MMP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 10 (MMP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle MMP10 ELISA Kits anzeigen
    MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))
    Andere Bezeichnung
    Matrix Metalloproteinase 10 (MMP10 Produkte)
    Synonyme
    SL-2 ELISA Kit, STMY2 ELISA Kit, AV377895 ELISA Kit, MMP-10 ELISA Kit, matrix metallopeptidase 10 ELISA Kit, MMP10 ELISA Kit, Mmp10 ELISA Kit
    Hintergrund

    Gene Name: Matrix Metalloproteinase 10

    Gene Aliases: STMY2, SL2, Stromelysin 2, Transin-2

    Gen-ID
    17384
    UniProt
    O55123
Sie sind hier:
Kundenservice