MVP ELISA Kit

Produktnummer ABIN5656626

Produktdetails MVP ELISA Kit

Target: MVP (Major Vault Protein (MVP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Major Vault Protein (MVP). No significant cross-reactivity or interference between Major Vault Protein (MVP) and analogues was observed.

Sensitivität: 0.065 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Vault Protein (MVP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Vault Protein (MVP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Vault Protein (MVP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Vault Protein (MVP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Vault Protein (MVP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Vault Protein (MVP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MVP

Target: MVP (Major Vault Protein (MVP))

Andere Bezeichnung: Major Vault Protein (MVP Produkte)

Synonyme: LRP ELISA Kit, VAULT1 ELISA Kit, 2310009M24Rik ELISA Kit, cb771 ELISA Kit, wu:fb52b05 ELISA Kit, wu:fc02g01 ELISA Kit, mvp ELISA Kit, MGC145641 ELISA Kit, Tb05.45E22.810 ELISA Kit, major vault protein ELISA Kit, major vault protein L homeolog ELISA Kit, putative major vault protein ELISA Kit, MVP ELISA Kit, Mvp ELISA Kit, mvp ELISA Kit, mvp.L ELISA Kit, Tc00.1047053510353.10 ELISA Kit, Tb927.5.4460 ELISA Kit, Tb10.70.0520 ELISA Kit, Tb10.70.5840 ELISA Kit, LMJF_05_0060 ELISA Kit

Hintergrund:

Gene Name: Major Vault Protein

Gene Aliases: LRP, VAULT1, Lung resistance-related protein

Gen-ID: 9961

UniProt: Q14764