IDH1 ELISA Kit

Produktnummer ABIN5656120

Produktdetails IDH1 ELISA Kit

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.

Sensitivität: 0.37 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isocitrate Dehydrogenase 1, Soluble (IDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isocitrate Dehydrogenase 1, Soluble (IDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: Isocitrate Dehydrogenase 1, Soluble (IDH1 Produkte)

Synonyme: IDCD ELISA Kit, IDH ELISA Kit, IDP ELISA Kit, IDPC ELISA Kit, PICD ELISA Kit, NADP-CICDH ELISA Kit, AI314845 ELISA Kit, AI788952 ELISA Kit, E030024J03Rik ELISA Kit, Id-1 ELISA Kit, Idh-1 ELISA Kit, Idpc ELISA Kit, cb876 ELISA Kit, fm90e09 ELISA Kit, im:7143416 ELISA Kit, wu:fm90e09 ELISA Kit, F23E12.180 ELISA Kit, F23E12_180 ELISA Kit, IDH-I ELISA Kit, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 ELISA Kit, isocitrate dehydrogenase 1 ELISA Kit, isocitrate dehydrogenase I ELISA Kit, isocitrate dehydrogenase (NADP(+)) 1, cytosolic ELISA Kit, isocitrate dehydrogenase 1 (NADP+), soluble ELISA Kit, isocitrate dehydrogenase 1 (NADP+) L homeolog ELISA Kit, isocitrate dehydrogenase 1 ELISA Kit, IDH1 ELISA Kit, Idh1 ELISA Kit, idh1.L ELISA Kit, idh1 ELISA Kit

Hintergrund:

Gene Name: Isocitrate Dehydrogenase 1, Soluble

Gene Aliases: PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase

Gen-ID: 3417

UniProt: O75874

Pathways: Warburg Effekt