Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IL-27 ELISA Kit

IL27 Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655929
  • Target Alle IL-27 (IL27) ELISA Kits anzeigen
    IL-27 (IL27) (Interleukin 27 (IL27))
    Reaktivität
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Interleukin 27 (IL27). No significant cross-reactivity or interference between Interleukin 27 (IL27) and analogues was observed.
    Sensitivität
    6.1 pg/mL
    Featured
    Zu unserem meistverkauften IL27 ELISA Kit
    Top Product
    Discover our top product IL27 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 27 (IL27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 27 (IL27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 27 (IL27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 27 (IL27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 27 (IL27) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 27 (IL27) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle IL-27 (IL27) ELISA Kits anzeigen
    IL-27 (IL27) (Interleukin 27 (IL27))
    Andere Bezeichnung
    Interleukin 27 (IL27 Produkte)
    Synonyme
    IL-27 ELISA Kit, IL-27A ELISA Kit, IL27A ELISA Kit, IL27p28 ELISA Kit, IL30 ELISA Kit, p28 ELISA Kit, RGD1561420 ELISA Kit, IL27 ELISA Kit, IL-27p28 ELISA Kit, Il30 ELISA Kit, interleukin 27 ELISA Kit, IL27 ELISA Kit, Il27 ELISA Kit
    Hintergrund

    Gene Name: Interleukin 27

    Gene Aliases: p28, IL30, Interleukin 30, Interleukin-27 subunit alpha

Sie sind hier:
Kundenservice