Granulin ELISA Kit

Produktnummer ABIN5654795

Produktdetails Granulin ELISA Kit

Target: Granulin (GRN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Granulin (GRN). No significant cross-reactivity or interference between Granulin (GRN) and analogues was observed.

Sensitivität: 0.64 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulin (GRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granulin (GRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulin (GRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granulin (GRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulin (GRN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulin (GRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Granulin

Target: Granulin (GRN)

Andere Bezeichnung: Granulin (GRN Produkte)

Synonyme: GRN ELISA Kit, grn ELISA Kit, wu:fb11g12 ELISA Kit, wu:fj18f05 ELISA Kit, MGC53354 ELISA Kit, DKFZp459H066 ELISA Kit, CLN11 ELISA Kit, GEP ELISA Kit, GP88 ELISA Kit, PCDGF ELISA Kit, PEPI ELISA Kit, PGRN ELISA Kit, epithelin ELISA Kit, acrogranin ELISA Kit, granulin precursor ELISA Kit, granulin a ELISA Kit, Granulins ELISA Kit, granulin L homeolog ELISA Kit, granulin ELISA Kit, GRN ELISA Kit, grna ELISA Kit, grn ELISA Kit, grn.L ELISA Kit, granulin ELISA Kit, ORF1 granulin ELISA Kit, Grn ELISA Kit

Substanzklasse: Viral Protein

Hintergrund:

Gene Name: Granulin

Gene Aliases: GEP, GP88, PCDGF, PEPI, PGRN, Proepithelin, Acrogranin, Glycoprotein of 88 Kda, Paragranulin

Gen-ID: 2896

UniProt: P28799