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GOLM1 ELISA Kit

GOLM1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5654788
  • Target Alle GOLM1 ELISA Kits anzeigen
    GOLM1 (Golgi Membrane Protein 1 (GOLM1))
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.94 ng/mL - 60 ng/mL
    Untere Nachweisgrenze
    0.94 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Golgi Protein 73 (GP73). No significant cross-reactivity or interference between Golgi Protein 73 (GP73) and analogues was observed.
    Sensitivität
    0.37 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi Protein 73 (GP73). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Golgi Protein 73 (GP73). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgi Protein 73 (GP73), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Golgi Protein 73 (GP73) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle GOLM1 ELISA Kits anzeigen
    GOLM1 (Golgi Membrane Protein 1 (GOLM1))
    Andere Bezeichnung
    Golgi Protein 73 (GOLM1 Produkte)
    Synonyme
    C9orf155 ELISA Kit, GOLPH2 ELISA Kit, GP73 ELISA Kit, PSEC0257 ELISA Kit, bA379P1.3 ELISA Kit, 2310001L02Rik ELISA Kit, AW125446 ELISA Kit, D030064E01Rik ELISA Kit, Golph2 ELISA Kit, GOLM1 ELISA Kit, golph2 ELISA Kit, gp73 ELISA Kit, golgi membrane protein 1 ELISA Kit, SPATA31 subfamily D, member 3 ELISA Kit, Golgi membrane protein 1 ELISA Kit, golgi membrane protein 1 L homeolog ELISA Kit, GOLM1 ELISA Kit, Golm1 ELISA Kit, Spata31d3 ELISA Kit, golm1 ELISA Kit, LOC100400549 ELISA Kit, golm1.L ELISA Kit
    Hintergrund

    Gene Name: Golgi Protein 73

    Gene Aliases: GOLM1, GOLPH2, C9orf155, Golgi Membrane Protein 1, Golgi Phosphoprotein 2

    Gen-ID
    105348
    UniProt
    Q91XA2
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