SERPINE2 ELISA Kit

Produktnummer ABIN5654565

Produktdetails SERPINE2 ELISA Kit

Target: SERPINE2 (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 2 (SERPINE2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glia Derived Nexin (GDN). No significant cross-reactivity or interference between Glia Derived Nexin (GDN) and analogues was observed.

Sensitivität: 0.135 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glia Derived Nexin (GDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glia Derived Nexin (GDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glia Derived Nexin (GDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glia Derived Nexin (GDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SERPINE2

Target: SERPINE2 (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 2 (SERPINE2))

Andere Bezeichnung: Glia Derived Nexin (SERPINE2 Produkte)

Synonyme: GDN ELISA Kit, GDNPF ELISA Kit, PI-7 ELISA Kit, PI7 ELISA Kit, PN-1 ELISA Kit, PN1 ELISA Kit, PNI ELISA Kit, SERPINE2 ELISA Kit, MGC143011 ELISA Kit, Gdnpn1 ELISA Kit, Pn-1 ELISA Kit, Spin4 ELISA Kit, B230326M24Rik ELISA Kit, PAI-1 ELISA Kit, Spi4 ELISA Kit, zgc:55929 ELISA Kit, serpin family E member 2 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 2 ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 ELISA Kit, SERPINE2 ELISA Kit, serpine2 ELISA Kit, Serpine2 ELISA Kit

Hintergrund:

Gene Name: Glia Derived Nexin

Gene Aliases: SERPINE2, PI7, PN1, PNI, Protease Nexin I, Serpin Peptidase Inhibitor Clade E Member 2, Peptidase inhibitor 7, Protease nexin I

Gen-ID: 5270

UniProt: P07093