ENO2/NSE ELISA Kit

Produktnummer ABIN5653879

Produktdetails ENO2/NSE ELISA Kit

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Enolase, Neuron Specific (NSE). No significant cross-reactivity or interference between Enolase, Neuron Specific (NSE) and analogues was observed.

Sensitivität: 32 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase, Neuron Specific (NSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enolase, Neuron Specific (NSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enolase, Neuron Specific (NSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enolase, Neuron Specific (NSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: Enolase, Neuron Specific (ENO2 Produkte)

Synonyme: ENO2 ELISA Kit, DKFZp459B1817 ELISA Kit, NSE ELISA Kit, AI837106 ELISA Kit, D6Ertd375e ELISA Kit, Eno-2 ELISA Kit, RNEN3 ELISA Kit, eno3 ELISA Kit, wu:fc09h05 ELISA Kit, zgc:92418 ELISA Kit, enolase 2 ELISA Kit, enolase 2 (gamma, neuronal) ELISA Kit, enolase 2, gamma neuronal ELISA Kit, enolase 2, gamma, neuronal ELISA Kit, ENO2 ELISA Kit, Eno2 ELISA Kit, eno2 ELISA Kit

Hintergrund:

Gene Name: Enolase, Neuron Specific

Gene Aliases: ENO2, Enolase 2, Gamma Enolase, 2-phospho-D-glycerate hydro-lyase, Neural enolase

Gen-ID: 13807

UniProt: Q545V3