CLEC11A ELISA Kit
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- Target Alle CLEC11A ELISA Kits anzeigen
- CLEC11A (C-Type Lectin Domain Family 11, Member A (CLEC11A))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.94 ng/mL - 60 ng/mL
- Untere Nachweisgrenze
- 0.94 ng/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 11, Member A (CLEC11A). No significant cross-reactivity or interference between C-Type Lectin Domain Family 11, Member A (CLEC11A) and analogues was observed.
- Sensitivität
- 0.39 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle CLEC11A ELISA Kits anzeigen
- CLEC11A (C-Type Lectin Domain Family 11, Member A (CLEC11A))
- Andere Bezeichnung
- C-Type Lectin Domain Family 11, Member A (CLEC11A Produkte)
- Synonyme
- CLEC11A ELISA Kit, CLECSF3 ELISA Kit, LSLCL ELISA Kit, P47 ELISA Kit, SCGF ELISA Kit, AW457320 ELISA Kit, Clecsf3 ELISA Kit, Scgf ELISA Kit, C-type lectin domain containing 11A ELISA Kit, C-type lectin domain family 11, member a ELISA Kit, CLEC11A ELISA Kit, Clec11a ELISA Kit
- Hintergrund
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Gene Name: C-Type Lectin Domain Family 11, Member A
Gene Aliases: SCGF, P47, LSLCL, CLECSF3, Stem Cell Growth Factor, Lymphocyte Secreted C-Type Lectin, C-type lectin superfamily member 3
- Gen-ID
- 6320
- UniProt
- Q9Y240
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