BMPR1B ELISA Kit

Produktnummer ABIN5652299

Produktdetails BMPR1B ELISA Kit

Target: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1B (BMPR1B). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1B (BMPR1B) and analogues was observed.

Sensitivität: 0.117 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1B (BMPR1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 1B (BMPR1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu BMPR1B

Target: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Andere Bezeichnung: Bone Morphogenetic Protein Receptor 1B (BMPR1B Produkte)

Synonyme: ALK6 ELISA Kit, BMPR-IB ELISA Kit, FecB ELISA Kit, alk-6 ELISA Kit, alk6 ELISA Kit, cdw293 ELISA Kit, alk6b ELISA Kit, zgc:172219 ELISA Kit, BMPR1B ELISA Kit, AI385617 ELISA Kit, ALK-6 ELISA Kit, AV355320 ELISA Kit, Acvrlk6 ELISA Kit, Alk6 ELISA Kit, BMPR-1B ELISA Kit, CFK-43a ELISA Kit, SKR6 ELISA Kit, BR1b ELISA Kit, alk6tr ELISA Kit, bmpr1b ELISA Kit, zALK-6 ELISA Kit, zgc:92220 ELISA Kit, CDw293 ELISA Kit, BMP15 ELISA Kit, BMPRIB ELISA Kit, RPK-1 ELISA Kit, bone morphogenetic protein receptor type 1B ELISA Kit, bone morphogenetic protein receptor, type IBb ELISA Kit, bone morphogenetic protein receptor, type 1B ELISA Kit, bone morphogenetic protein receptor, type IBa ELISA Kit, BMPR1B ELISA Kit, bmpr1b ELISA Kit, bmpr1bb ELISA Kit, Bmpr1b ELISA Kit, bmpr1ba ELISA Kit

Hintergrund:

Gene Name: Bone Morphogenetic Protein Receptor 1B

Gene Aliases: CDw293, CD293, BMPR1-B, ALK6