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Annexin IV ELISA Kit

ANXA4 Reaktivität: Human Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Plasma, Serum
Produktnummer ABIN5651838
  • Target Alle Annexin IV (ANXA4) ELISA Kits anzeigen
    Annexin IV (ANXA4) (Annexin A4 (ANXA4))
    Reaktivität
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    18.75 pg/mL - 1200 pg/mL
    Untere Nachweisgrenze
    18.75 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Annexin A4 (ANXA4). No significant cross-reactivity or interference between Annexin A4 (ANXA4) and analogues was observed.
    Sensitivität
    7.4 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A4 (ANXA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A4 (ANXA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A4 (ANXA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A4 (ANXA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Annexin IV (ANXA4) ELISA Kits anzeigen
    Annexin IV (ANXA4) (Annexin A4 (ANXA4))
    Andere Bezeichnung
    Annexin A4 (ANXA4 Produkte)
    Synonyme
    ANXA4 ELISA Kit, Anx4 ELISA Kit, XAnx4 ELISA Kit, pig28 ELISA Kit, X-anx4 ELISA Kit, Xanx-4 ELISA Kit, ANXIV ELISA Kit, anx4 ELISA Kit, ANX4 ELISA Kit, PIG28 ELISA Kit, ZAP36 ELISA Kit, Annexin-4 ELISA Kit, P32.5 ELISA Kit, PAP-II ELISA Kit, PP4-X ELISA Kit, AI265406 ELISA Kit, AIV ELISA Kit, AW106930 ELISA Kit, annexin A4 ELISA Kit, annexin A4 L homeolog ELISA Kit, ANXA4 ELISA Kit, anxa4 ELISA Kit, CC1G_00713 ELISA Kit, LOC100280951 ELISA Kit, Anxa4 ELISA Kit, anxa4.L ELISA Kit
    Hintergrund

    Gene Name: Annexin A4

    Gene Aliases: ANX-A4, ANX4, PIG28, 35-beta calcimedin, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, Placental anticoagulant protein II

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