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Annexin A2 ELISA Kit

ANXA2 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5651833
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    Annexin A2 (ANXA2)
    Reaktivität
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    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.
    Sensitivität
    0.061 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A2 (ANXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A2 (ANXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A2 (ANXA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Annexin A2 (ANXA2) ELISA Kits anzeigen
    Annexin A2 (ANXA2)
    Andere Bezeichnung
    Annexin A2 (ANXA2 Produkte)
    Synonyme
    ANX2 ELISA Kit, ANX2L4 ELISA Kit, CAL1H ELISA Kit, LIP2 ELISA Kit, LPC2 ELISA Kit, LPC2D ELISA Kit, P36 ELISA Kit, PAP-IV ELISA Kit, AW215814 ELISA Kit, Cal1h ELISA Kit, p36 ELISA Kit, anx2 ELISA Kit, lip2 ELISA Kit, lpc2 ELISA Kit, cal1h ELISA Kit, lpc2d ELISA Kit, pap-i ELISA Kit, anx2l4 ELISA Kit, MGC76145 ELISA Kit, ANXA2 ELISA Kit, anxa2 ELISA Kit, anxa2-a ELISA Kit, anxa2-b ELISA Kit, annexin A2 ELISA Kit, annexin A2 S homeolog ELISA Kit, ANXA2 ELISA Kit, Anxa2 ELISA Kit, anxa2 ELISA Kit, anxa2.S ELISA Kit
    Hintergrund

    Gene Name: Annexin A2

    Gene Aliases: ANX-A2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, Protein I, Chromobindin-8, Calpactin-1 heavy chain, Lipocortin II, Placental anticoagulant protein IV

    Gen-ID
    302
    UniProt
    P07355
    Pathways
    S100 Proteine
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