alpha 2 Macroglobulin ELISA Kit

Produktnummer ABIN5651632

Produktdetails alpha 2 Macroglobulin ELISA Kit

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12.5 ng/mL - 800 ng/mL

Untere Nachweisgrenze: 12.5 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Urine

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.

Sensitivität: 5.9 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin (a2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin (a2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Andere Bezeichnung: Alpha-2-Macroglobulin (A2M Produkte)

Synonyme: A2MD ELISA Kit, CPAMD5 ELISA Kit, FWP007 ELISA Kit, S863-7 ELISA Kit, A2MAC1 ELISA Kit, A2m1 ELISA Kit, A2maa ELISA Kit, Mam ELISA Kit, A2mp ELISA Kit, A2M ELISA Kit, LOC733429 ELISA Kit, endod ELISA Kit, cpamd5 ELISA Kit, fwp007 ELISA Kit, s863-7 ELISA Kit, a2mb ELISA Kit, endodermin ELISA Kit, Alpha-2-M ELISA Kit, alpha-2-macroglobulin ELISA Kit, pregnancy-zone protein L homeolog ELISA Kit, alpha-2-macroglobulin S homeolog ELISA Kit, pregnancy zone protein ELISA Kit, alpha-2-macroglobulin-like ELISA Kit, A2M ELISA Kit, A2m ELISA Kit, LOC477699 ELISA Kit, pzp.L ELISA Kit, AZL_c00450 ELISA Kit, LOC100349077 ELISA Kit, a2m ELISA Kit, LOC100061656 ELISA Kit, LOC100090399 ELISA Kit, a2m.S ELISA Kit, LOC100469973 ELISA Kit, LOC100595735 ELISA Kit, PZP ELISA Kit, LOC100353095 ELISA Kit, LOC101122940 ELISA Kit, LOC101801552 ELISA Kit, LOC100911545 ELISA Kit

Hintergrund:

Gene Name: Alpha-2-Macroglobulin

Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Gen-ID: 100911545

UniProt: P06238

Pathways: Lipid Metabolism