Intestinal Alkaline Phosphatase ELISA Kit

Produktnummer ABIN5651582

Produktdetails Intestinal Alkaline Phosphatase ELISA Kit

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Intestinal (ALPI). No significant cross-reactivity or interference between Alkaline Phosphatase, Intestinal (ALPI) and analogues was observed.

Sensitivität: 0.65 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Intestinal (ALPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Intestinal (ALPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: Alkaline Phosphatase, Intestinal (ALPI Produkte)

Synonyme: IAP ELISA Kit, Alp1 ELISA Kit, S51097 ELISA Kit, 2010001C14Rik ELISA Kit, Akp6 ELISA Kit, gIAP ELISA Kit, alpi ELISA Kit, im:7149588 ELISA Kit, si:ch211-262h13.1 ELISA Kit, wu:fa95f04 ELISA Kit, zgc:110794 ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, alkaline phosphatase, intestinal, tandem duplicate 1 ELISA Kit, intestinal-type alkaline phosphatase ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alpi.1 ELISA Kit, LOC100724954 ELISA Kit

Hintergrund:

Gene Name: Alkaline Phosphatase, Intestinal

Gene Aliases: CIP, IAP, Calf Intestinal Alkaline Phosphatase

Gen-ID: 248

UniProt: P09923