Alkaline Phosphatase ELISA Kit

Produktnummer ABIN5651576

Produktdetails Alkaline Phosphatase ELISA Kit

Target: Alkaline Phosphatase (ALP)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP). No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.

Sensitivität: 0.064 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase (ALP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase (ALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase (ALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase (ALP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase (ALP Produkte)

Synonyme: ALP ELISA Kit, ALP1 ELISA Kit, CRASH ELISA Kit, ECK0378 ELISA Kit, JW0374 ELISA Kit, psiA ELISA Kit, 2410004D18Rik ELISA Kit, AU040643 ELISA Kit, AW060375 ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, Akp2 ELISA Kit, alp ELISA Kit, zgc:56672 ELISA Kit, DDBDRAFT_0205491 ELISA Kit, DDBDRAFT_0231570 ELISA Kit, DDB_0205491 ELISA Kit, DDB_0231570 ELISA Kit, Actn2lp ELISA Kit, Alp ELISA Kit, asparaginase like 1 ELISA Kit, bacterial alkaline phosphatase ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase ELISA Kit, PDZ and LIM domain 3 ELISA Kit, ASRGL1 ELISA Kit, phoA ELISA Kit, Asrgl1 ELISA Kit, CCL27 ELISA Kit, alpl ELISA Kit, alp ELISA Kit, Pdlim3 ELISA Kit

Hintergrund:

Gene Name: Alkaline Phosphatase

Gene Aliases: AKP, ALKP, Basic Phosphatase