Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

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Applikation
Flow Cytometry (FACS)
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Verwendungszweck Measurement of Autophagy processes - Screening compounds affecting Autophagy
Proben Adherent Cell Culture, Suspension Cell Culture
Produktmerkmale This Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells

Features and Benefits: Measurement of cellular autophaghy processes
Bestandteile Assay Buffer (10X)
Autophagy Stain (500X)
Cell Death Marker (500X)
Positive Control Reagent (10000X)
Hintergrund Autophagy or autophagocytosis (""self-eating"") refers to a process of degradation of cytoplasmic components within lysosomes, a unique process mediated by autophagosomes. Autophagy consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. Autophagosomes engulf a portion of cytoplasm, thus autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases.
Applikationshinweise Measurement of Autophagy processes - Screening compounds affecting Autophagy
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Haltbarkeit 12 months