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NRF2 ELISA Kit

NFE2L2 Reaktivität: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Produktnummer ABIN5526730
  • Target Alle NRF2 (NFE2L2) ELISA Kits anzeigen
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Reaktivität
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    DNA-Binding ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human NRF2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NRF2 binding sequences to semi-quantitatively detect active NRF2 in lysates or nuclear extracts. Only available in North America.
    Proben
    Cell Lysate, Nuclear Extract
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The olionucleotide/antibody pair provided in this kit recognizes human NRF2 in whole lysates and nuclear extracts.
    Produktmerkmale
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Bestandteile
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target Alle NRF2 (NFE2L2) ELISA Kits anzeigen
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Andere Bezeichnung
    Nrf2 (NFE2L2 Produkte)
    Synonyme
    Nrf2 ELISA Kit, nfe2l2 ELISA Kit, wu:fc15g09 ELISA Kit, wu:fj67e03 ELISA Kit, NF-E2R2 ELISA Kit, NRF2 ELISA Kit, AI194320 ELISA Kit, nuclear factor, erythroid 2-like 2a ELISA Kit, nuclear factor, erythroid 2 like 2 S homeolog ELISA Kit, nuclear factor, erythroid 2 like 2 ELISA Kit, nuclear factor, erythroid derived 2, like 2 ELISA Kit, nuclear factor, erythroid 2-like 2 ELISA Kit, nfe2l2a ELISA Kit, nfe2l2.S ELISA Kit, NFE2L2 ELISA Kit, Nfe2l2 ELISA Kit
    Gen-ID
    4780
    UniProt
    Q16236
    Pathways
    ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
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