TFEB ELISA Kit

Produktnummer ABIN5526704

Produktdetails TFEB ELISA Kit

Target: TFEB (Transcription Factor EB (TFEB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: DNA-Binding ELISA

Applikation: ELISA

Verwendungszweck: Human TFEB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical TFEB binding sequences to semi-quantitatively detect active TFEB in lysates or nuclear extracts. Only available in North America.

Proben: Cell Lysate, Nuclear Extract

Analytische Methode: Semi-Quantitative

Spezifität: The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.

Produktmerkmale:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Bestandteile:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für TFEB ELISA Kit (ABIN5526704)

Mlejnek, Havlasek, Pastvova, Dolezel, Dostalova: "Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 153, pp. 113328, (2022) (PubMed).

Kim, Kim, Kim, Kim, Han, Byeon, Lee, Kim: "HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer." in: Autophagy, Vol. 14, Issue 3, pp. 385-403, (2019) (PubMed).

Details zu TFEB

Target: TFEB (Transcription Factor EB (TFEB))

Andere Bezeichnung: TFEB (TFEB Produkte)

Synonyme: TFEB ELISA Kit, tcfeb ELISA Kit, MGC145796 ELISA Kit, alphatfeb ELISA Kit, ALPHATFEB ELISA Kit, BHLHE35 ELISA Kit, TCFEB ELISA Kit, Tcfeb ELISA Kit, bHLHe35 ELISA Kit, transcription factor EB ELISA Kit, TFEB ELISA Kit, tfeb ELISA Kit, Tfeb ELISA Kit

Gen-ID: 7942

UniProt: P19484