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C-Peptide CLIA Kit

Reaktivität: Human Chemiluminescent Sandwich ELISA
Produktnummer ABIN504796
  • Target Alle C-Peptide CLIA Kits anzeigen
    C-Peptide
    Reaktivität
    • 5
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    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Analytische Methode
    Quantitative
    Produktmerkmale
    The Quantitative Determination of Circulating C-Peptide Concentrations in Human Serum by a Microplate Chemiluminescence immunoassay
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  • Applikationshinweise
    All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.
    Probenmenge
    50 μL
    Plattentyp
    Pre-coated
    Protokoll

    Specimien Collection and Preparation:

    The specimens shall be blood serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain red-top venipuncture tube without additives or gel barrier. Allow the blood to clot. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2_x001E_8(C for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20(C for up to 30 days. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.100ml of the specimen is required.

    Reagent Preparation:

    1. Wash Buffer Dilute contents of Wash Concentrate to 1000ml with distilled or deionized water in a suitable storage container. Store at room temperature (20-27(C) for up to 60 days. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly.

    Test Procedure:

    Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20 - 27( C). 1. Format the microplates wells for calibrator, control and patient specimen to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.050 ml (50l) of the appropriate calibrators, controls and samples into the assigned wells. 3. Add 0.100 ml (100l) of the C-Peptide Tracer Reagent to each well. It is very important to dispense all reagents close to the bottom of the microwell. 4. Swirl the microplate gently for 20-30 seconds to mix. Cover with a plastic wrap. 5. Incubate for 60 minutes at room temperature 6. Discard the contents of the microplate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper. 7. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is used, fill each well to the top by squeezing the container. Avoiding air bubbles. Decant the wash and repeat four (4) additional times. Add 0.100 ml (100l) of working signal reagent solution to all wells (see Reagent Preparation Section). Always add reagents in the same order to minimize reaction time differences between wells. Incubate at room temperature for five (5) minutes in the dark. 10. Read the RLUs (Relative Light Units) using a 96 well microplate luminometer for 0.2 1.0 seconds per well. The results should be read within thirty (30) minutes of adding the stop solution.
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  • Target Alle C-Peptide CLIA Kits anzeigen
    C-Peptide
    Abstract
    C-Peptide Produkte
    Synonyme
    insulin 2 CLIA Kit, Ins2 CLIA Kit
    Hintergrund
    Diabetes is one of the leading causes of disability and death in the U.S. It affects an estimated 16 million Americans, about one third of them do not even know they have the disease. The causes of diabetes are not precisely known, but both genetic and environmental factors play a significant role. The disease is marked by deficiencies in the bodys ability to produce and properly use insulin. The most common forms of diabetes are type 1, in which the bodys ability to produce insulin is destroyed, and type 2, in which the body is resistant to insulin even though some amount of insulin may be produced. In-vitro determination of insulin and C-Peptide levels help in the differential diagnosis of liver disease, acromegaly, Cushings syndrome, familial glucose intolerance, insulinoma, renal failure, ingestion of accidental oral hypoglycemic drugs or insulin induced factitious hypoglycemia. Both insulin and C-Peptide are produced by enzymatic cleavage of proinsulin. Proinsulin is stored in the secretory granules of pancreatic (-cells and is split into a 31 amino acid connecting peptide (C-Peptide, MW 3600) and insulin (MW 6000). C-Peptide is devoid of any biological activity but appears to be necessary to maintain the structural integrity of insulin. Although insulin and C-Peptide are secreted into portal circulation in equimolar concentrations, fasting levels of C-Peptide are 5-10 folds higher than those of insulin owing to the longer half-life of C-Peptide. The liver does not extract C-Peptide however, it is removed from the circulation by degradation in the kidneys with a fraction passing out unchanged in urine. Hence urine C-Peptide levels correlate well with fasting C-Peptide levels in serum. The glucagon stimulated C-Peptide determination is often used for differential diagnosis of insulin-dependent from non-insulin-dependent diabetic patients.
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