SMAD5 ELISA Kit (SMAD, Mothers Against DPP Homolog 5)

Details for Product SMAD5 ELISA Kit No. ABIN4886297, Anbieter: Anmelden zum Anzeigen
Antigen
  • SBN
  • fb67b04
  • mad
  • madh5
  • sb:cb400
  • smd5
  • wu:fb67b04
  • XSmad4alpha
  • dpc4
  • jip
  • jv5-1
  • madh4
  • smad10
  • smad5
  • xsmad4
  • DWFC
  • JV5-1
  • MADH5
  • 1110051M15Rik
  • AI451355
  • Dwf-C
  • Madh5
  • MusMLP
  • SMAD5
  • DKFZp468M0619
  • SMAD family member 5
  • MAD homolog 5 (Drosophila)
  • SMAD family member 4, gene 2
  • SMAD5
  • smad5
  • smad4.2-b
  • Smad5
Epitop
pSer463, pSer465
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
4
1
1
1
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
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Verwendungszweck Human Phospho-SMAD5 (SER463/465) ELISA Kit. This assay semi-quantitatively measures phophorylated SMAD5 (Ser463/465) in lysate samples.
Proben Cell Lysate, Tissue Lysate
Analytische Methode Semi-Quantitative
Nachweismethode Colorimetric
Spezifität The antibody pair provided in this kit recognizes Human SMAD5 phosphorylated at site Serine-463/465.
Produktmerkmale
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Andere Bezeichnung SMAD5 (SMAD5 ELISA Kit Abstract)
Gen-ID 4090
UniProt Q99717
Forschungsgebiet Transcription Factors, Signaling, Organogenesis
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Informationen zur Lagerung Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit 6 months