Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ST8SIA2 ELISA Kit

ST8SIA2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884671
  • Target Alle ST8SIA2 ELISA Kits anzeigen
    ST8SIA2 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 2 (ST8SIA2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human ST8SIA2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ST8SIA2 ELISA Kits anzeigen
    ST8SIA2 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 2 (ST8SIA2))
    Andere Bezeichnung
    ST8SIA2 (ST8SIA2 Produkte)
    Synonyme
    cb394 ELISA Kit, id:ibd5161 ELISA Kit, siat8 ELISA Kit, SIAT8B ELISA Kit, HsT19690 ELISA Kit, ST8SIA-II ELISA Kit, STX ELISA Kit, SIAT 8B ELISA Kit, AI323367 ELISA Kit, ST8SiaII ELISA Kit, Siat8b ELISA Kit, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 ELISA Kit, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 L homeolog ELISA Kit, st8sia2 ELISA Kit, st8sia2.L ELISA Kit, ST8SIA2 ELISA Kit, St8sia2 ELISA Kit
    Gen-ID
    8128
    UniProt
    Q92186
Sie sind hier:
Kundenservice