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Activin A Receptor Type IB/ALK-4 ELISA Kit

ACVR1B Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881724
  • Target Alle Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA Kits anzeigen
    Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
    Reaktivität
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Activin R1B (ALK-4) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes human Activin R1B. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ACVR1B ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA Kits anzeigen
    Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
    Andere Bezeichnung
    Activin R1B (ACVR1B Produkte)
    Synonyme
    ACVR1B ELISA Kit, alk4 ELISA Kit, skr2 ELISA Kit, alk-4 ELISA Kit, xalk4 ELISA Kit, actrib ELISA Kit, acvrlk4 ELISA Kit, DKFZp469A1614 ELISA Kit, 6820432J04 ELISA Kit, ActR-IB ELISA Kit, ActRIB ELISA Kit, Acvrlk4 ELISA Kit, Alk4 ELISA Kit, SKR2 ELISA Kit, ACTRIB ELISA Kit, ACVRLK4 ELISA Kit, ALK4 ELISA Kit, Skr2 ELISA Kit, axis ELISA Kit, taram-a ELISA Kit, tarama ELISA Kit, wu:fb75f01 ELISA Kit, activin A receptor type 1B ELISA Kit, Activin receptor type-1B ELISA Kit, activin A receptor, type IB ELISA Kit, activin A receptor, type 1B ELISA Kit, activin A receptor type 1Ba ELISA Kit, ACVR1B ELISA Kit, acvr1b ELISA Kit, acv1b ELISA Kit, Acvr1b ELISA Kit, acvr1ba ELISA Kit
    Gen-ID
    91
    UniProt
    P36896
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