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Albumin ELISA Kit

ALB Reaktivität: Maus Colorimetric Competition ELISA Urine
Produktnummer ABIN454013
  • Target Alle Albumin (ALB) ELISA Kits anzeigen
    Albumin (ALB)
    Reaktivität
    • 10
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Untere Nachweisgrenze
    0.1 µg/mL
    Applikation
    ELISA
    Verwendungszweck
    This ELISA allows quantification of albumin in mouse urine, so that an estimation of kidney function is possible.
    Proben
    Urine
    Analytische Methode
    Quantitative
    Produktmerkmale
    Determination of albumin is carried out as direct competitive ELISA.
    Featured
    Zu unserem meistverkauften ALB ELISA Kit
    Top Product
    Discover our top product ALB ELISA Kit
  • Plattentyp
    Pre-coated
    Protokoll
    Mouse albumin has been pre-coated onto a microtiter plate. During incubation binding of an enzyme-linked anti-albumin(mouse)-antibody is inhibited by albumin from samples/ standards. After washing away any unbound antibody a substrate solution is added. Developing colour correlated with the amount of antibody conjugate. Absorption at 450 nm is reverse proportional to albumin concentration.
    Testdurchführung

    Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 90 min with shaking,120 min without shaking.
    Wash Substrate incubation: 100 µL/well 10 min, room temperature.
    Add stop solution 100 µL/well.
    Read at 450 nm

    Testpräzision
    Intraassay precision (CV):(sample dilution 1:100, n=12) at 3.1 µg/ml: 14.3%at 30.4 µg/ml: 6.1% at 223 µg/ml: 5.8%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Liebisch, Bondeva, Franke, Hause, Wolf: "Growth arrest specific 2-like protein 1 expression is upregulated in podocytes through advanced glycation end-products." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2016) (PubMed).

    Schordan, Grisk, Schordan, Miehe, Rumpel, Endlich, Giebel, Endlich: "OPN deficiency results in severe glomerulosclerosis in uninephrectomized mice." in: American journal of physiology. Renal physiology, (2013) (PubMed).

    Lee, Auger, Park, Kang, Oak, Kim, Schini-Kerth: "An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice." in: PLoS ONE, Vol. 8, Issue 6, pp. e65227, (2013) (PubMed).

    Hyvoenen, Saurus, Wasik, Heikkilae, Havana, Trokovic, Saleem, Holthoefer, Lehtonen: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).

    Heikkilae, Juhila, Lassila, Messing, Peraelae, Lehtonen, Lehtonen, Sjef Verbeek, Holthofer: "beta-Catenin mediates adriamycin-induced albuminuria and podocyte injury in adult mouse kidneys." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2437-46, (2010) (PubMed).

  • Target Alle Albumin (ALB) ELISA Kits anzeigen
    Albumin (ALB)
    Andere Bezeichnung
    Albumin (ALB Produkte)
    Synonyme
    PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit
    Hintergrund
    When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages. In examining nephrotoxic substances and therapeutic strategies regarding kidney damages, animal models are frequently used.
    Pathways
    Lipid Metabolism
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