CaspGLOWTM Fluorescein Active Caspase-2 Staining Kit

Details zu Produkt Nr. ABIN412025, Anbieter: Anmelden zum Anzeigen
Antigen
  • xCaspase-2
  • Caspase-2
  • ICH-1
  • Nedd2
  • CASP-2
  • ICH1
  • NEDD-2
  • NEDD2
  • PPP1R57
  • ICH-1L/1S
  • ICH1L1S
  • caspase 2
  • caspase 2 L homeolog
  • caspase-2
  • CASP2
  • casp2.L
  • CpipJ_CPIJ008254
  • Casp2
Alternativen
Human Caspase 2 ELISA Kit
Reaktivität
Säugetier
7
6
3
1
1
1
1
1
1
1
1
1
1
Applikation
Detection (D), Fluorescence Microscopy (FM), Flow Cytometry (FACS)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck The CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit provides a convenient means for sensitive detection of activated caspase-2 in living cells. The assay utilizes the caspase-2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-2 in apoptotic cells.
Marke CaspGLOW™
Proben Cell Samples
Nachweismethode Fluorometric
Spezifität The CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit provides a convenient means for sensitive detection of activated caspase-2 in living cells. The assay utilizes the caspase-2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-2 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Produktmerkmale CaspGLOWTM Fluorescein Active Caspase-2 Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspases-2 in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader.
Bestandteile FITC-VDVAD-FMK
Wash Buffer
Z-VAD-FMK
Andere Bezeichnung Caspase-2 (CASP2 ELISA Kit Abstract)
Hintergrund Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit provides a convenient means for sensitive detection of activated caspase-2 in living cells. The assay utilizes the caspase-2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-2 in apoptotic cells.
Forschungsgebiet Apoptosis/Necrosis, Cancer, Autophagy
Applikationshinweise Sensitive detection of activated caspase-2 in living cells.
Kommentare

Further details regarding sample type: Live cells

Protokoll A. Staining Procedure:
1. Induce apoptosis in cells (1 x 10^6 /mL) by desired method. Concurrently incubate a control culture without induction. An additional negative control can be prepared by adding the caspase inhibitor Z-VAD-FMK at 1 µL/mL to an induced culture to inhibit caspase-2 activation.
2. Aliquot 300 µL each of the induced and control cultures into eppendorf tubes.
3. Add 1 µL of FITC-VDVAD-FMK into each tube and incubate for 0.5-1 hour at 37 °C incubator with 5 % CO 2.
4. Centrifuge cells at 3000 rpm for 5 minutes and remove supernatant.
5. Resuspend cells in 0.5 mL of Wash Buffer, and centrifuge again.
6. Repeat Step
5. Proceed to B, C, or D depending on methods of analysis. B. Quantification by Flow Cytometry: For flow cytometric analysis, resuspend cells in 300 µL of Wash Buffer. Keep samples on ice. Analyzing samples by flow cytometry using the FL-1 channel. C. Detection by Fluorescence Microscopy: For fluorescence microscopic analysis, resuspend cells in 100 µL Wash Buffer. Transfer one drop of the cell suspension onto a microslide and cover with a coverslip. Observe cells under a fluorescence microscope using FITC filter. Caspase positive cells appear to have brighter green signals, whereas caspase negative control cells show much weaker signal. D. Analysis by Fluorescence Plate Reader: For analysis with fluorescence plate reader, resuspend cells in 100 µL Wash Buffer and then transfer the cell suspension into each well in the black microtiter plate. Measure the fluorescence intensity at Ex. = 485 nm and Em. = 535 nm. For control, use wells containing unlabeled cells.
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung -20 °C
Haltbarkeit 6-12 months
Produkt verwendet in: Espín, Roca, Candel, Sepulcre, González-Rosa, Alcaraz-Pérez, Meseguer, Cayuela, Mercader, Mulero: "TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3." in: Disease models & mechanisms, Vol. 6, Issue 2, pp. 383-96, 2013 (PubMed).

Ito, Yip, Katz, Fonseca, Hedley, Chow, Xu, Wood, Bastianutto, Schimmer, Kelley, Liu: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed).

Yip, Ito, Mao, Au, Hedley, Mocanu, Bastianutto, Schimmer, Liu: "Potential use of alexidine dihydrochloride as an apoptosis-promoting anticancer agent." in: Molecular cancer therapeutics, Vol. 5, Issue 9, pp. 2234-40, 2006 (PubMed).

Abdel-Latif, Murray, Renberg, ONeill, Porter, Jensen, Johnson: "Cell death in bovine parvovirus-infected embryonic bovine tracheal cells is mediated by necrosis rather than apoptosis." in: The Journal of general virology, Vol. 87, Issue Pt 9, pp. 2539-48, 2006 (PubMed).

Haben Sie etwas anderes gesucht?