SOD Activity Assay Kit

Antigen: SOD

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411902

Produktbeschreibung

Beschreibung: Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. The sensitive SOD assay kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Kit

Anwendungen

Protokoll: 1. Add 20 μl of Sample Solution to each sample and blank 2 well and add 20 μl H 2 O to each Blank 1 and Blank 3 well (See Table I). 2. Add 200 μl of the WST Working Solution to each well. 3. Add 20 μl of Dilution Buffer to each Blank 2 and Blank 3 well. 4. Add 20 μl of Enzyme Working solution to each sample and Blank 1 well, mix thoroughly.

Applikationshinweise: Note: since the superoxide will release immediately after the addition of Enzyme working Solution to each well, use a multiple channel pipette to avoid reaction time lag of each well. 5. Incubate plates at 37 o C for 20 minutes. 6. Read the absorbance at 450 nm using a microplate reader. 7. Calculate the SOD activity (inhibition rate%) using the following equation. (A blank1 – A blank3 ) – (A sample – A blank2 ) SOD Activity (inhibition rate%) = --------------------------------------------- x 100 (A blank1 – A blank3 ) Table 1: Amount of each solution for sample, blank1, 2, and 3 Component K335-100 Color Code Parts 100 assays Cap Color Number WST Solution 1 ml Red K335-100-1 SOD Enzyme Solution 20 μl Green K335-100-2 SOD Assay Buffer 20 ml WM K335-100-3 SOD Dilution Buffer 10 ml NM K335-100-4 0 20 40 60 80 100 0 0.1 1 5 10 SOD  Activity (U/ml) Pe rce n t  Inhi b itio n

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Mao, Zhong, Feng et al.: "Protective Effects of Paeoniflorin Against Glutamate-Induced Neurotoxicity in PC12 Cells via Antioxidant Mechanisms and Ca(2+) Antagonism." in: Cellular and molecular neurobiology, 2010 (PubMed).