Active Human Caspases Group III

Antigen: Caspase (CASP)

Synonyme: DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411796

Produktbeschreibung

Produktmerkmale: Unit definition: One unit of the active recombinant caspase-6,-8,-9 & -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10), and LEHD-pNA (for caspase-9), respectively, per hour at 37 o C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Weitere Bezeichnung: Caspase

Beschreibung: Caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The Group III recombinant caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10) and LEHD-pNA (for caspase-9), respectively.

Anwendungen

Applikationshinweise: Active recombinant caspases are useful for screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as positive controls in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to BioVision’s Fluorometric or Colorimetric Caspase Assay Kits.

Buffer: Lyophilized powder

Lagerung: The lyophilized recombinant caspases are stable for 1 year at –70 o C. Following reconstitution in PBS, these enzymes should be aliquoted and stored at –70 o C. Avoid multiple freeze/thaw cycles as activity might decrease. SCIENTIFIC. Reconstitution: Reconstitute to 1 unit per μ l in PBS containing 15% glycerol

Beschränkungen: Nur für Forschungszwecke einsetzbar