Active Human Caspases Set IV

Antigen: Caspase (CASP)

Synonyme: DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370

Reaktivität: Human

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411793

Produktbeschreibung

Produktmerkmale: One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, WEHD-pNA for caspase-4 and –5, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8 and –10 and LEHD-pNA for caspase-9) per hour at 37 o C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Weitere Bezeichnung: Caspase

Immunogen: Source: E. coli

Anwendungen

Applikationshinweise: Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics, determining substrate specificity, and as a positive control in caspase activity assays. For a complete caspase activity assay protocol, please refer to BioVision’s Fluorometric or Colorimetric Caspase Activity Assay Kits.

Buffer: Lyophilized powder STORAGE: Store at –70 o C. Stable for 6-12 months. PACKAGE CONTENT: Description Amount Cap color Active Recombinant Caspase-1 25 units Yellow Active Recombinant Caspase-2 25 units Orange Active Recombinant Caspase-3 25 units Red Active

Lagerung: Reconstitution: The active recombinant caspases can be reconstituted to 0.1-1 unit per μ l in PBS or the Reaction Buffer described above (available from BioVision, Cat.# 1068-20) for longer stability. We recommend using 1 unit per assay for analyzing caspase activity.

Beschränkungen: Nur für Forschungszwecke einsetzbar