Active Human Caspases Set III

Antigen: Caspase (CASP)

Synonyme: DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370

Reaktivität: Human

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411792

Produktbeschreibung

Produktmerkmale: One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8, LEHD-pNA for caspase-9, and IETD-pNA for caspase-10) per hour at 37 o C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Weitere Bezeichnung: Caspase

Immunogen: Source: E. coli

Anwendungen

Applikationshinweise: Highly active recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays. For a complete caspase activity assay protocol, please refer to BioVision’s Fluorometric or Colorimetric Caspase Activity Assay Kits.

Buffer: Lyophilized powder STORAGE: Store at –70 o C. Stable for 6-12 months. PACKAGE CONTENT: Description Amount Cap color Active Recombinant Caspase-1 25 units Yellow Active Recombinant Caspase-2 25 units Orange Active Recombinant Caspase-3 25 units Red Active

Lagerung: Reconstitution: The active recombinant caspases can be reconstituted to 0.1-1 unit per μ l in PBS or the Reaction Buffer described above (available from BioVision, Cat.# 1068-20) for longer stability. We recommend using 1 unit per assay for analyzing caspase activity.

Beschränkungen: Nur für Forschungszwecke einsetzbar