Active Human Caspases Set II

Antigen: Caspase (CASP)

Synonyme: DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370

Reaktivität: Human

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411791

Produktbeschreibung

Produktmerkmale: One unit of the recombinant human caspase-1, -2, -9, and -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA, VDVAD-pNA, LEHD-pNA, and IETD-pNA, respectively, per hour at 37 o C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Weitere Bezeichnung: Caspase

Beschreibung: Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes. Most of the pro-caspases are matured by proteolysis to yield large and small subunits. Active caspases are heterotetramers, each of which consists of two large and two small subunits.

Anwendungen

Applikationshinweise: Recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful for screen of caspase inhibitors, study enzyme kinetics and regulation, determine rtarget substrate specificity, and as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to BioVision’s Caspase Activity Assay Kits.

Buffer: Lyophilized powder

Lagerung: Reconstitution: Reconstituted to 0.1-1 unit per μ l in PBS. STORAGE: Store at –70 o C. Stable for 1 year at –70 o C

Beschränkungen: Nur für Forschungszwecke einsetzbar