Active Human Caspases Set I

Antigen: Caspase (CASP)

Synonyme: DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370

Reaktivität: Human

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411790

Produktbeschreibung

Produktmerkmale: One unit of the recombinant human caspase-3, -6, -7, and -8 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA, VEID-pNA, DEVD-pNA, and IETD-pNA, respectively, per hour at 37 o C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Weitere Bezeichnung: Caspase

Beschreibung: Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes and mature by proteolysis to yield large and small subunits. Active caspases are heterotetramers consisting of two large and two small subunits.

Anwendungen

Applikationshinweise: Highly active human caspases were expressed in E. coli and purified. Active caspases are useful for screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as a positive control in caspase activity assays and Western blot analysis. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to BioVision’s Caspase Activity Assay Kits.

Buffer: Lyophilized powder

Lagerung: Reconstitution: Reconstitute to 0.1-1 unit per μ l in PBS + 15% glycerol STORAGE: Store at –80 o C. Stable for 1 year at –80 o C BACKGROUND

Beschränkungen: Nur für Forschungszwecke einsetzbar