Aconitase Assay Kit

Antigen: Aconitase (ACO)

Synonyme: ACON-1, AconM, BEST:GH10550, CG9244, DmelCG9244, FBgn0010100, i204, i59, l(2)07054, m-acon, M-Acon, mAc, mAcon

Applikation: Enzymaktivitätsmessung (EAA)

Produktnummer: ABIN411780

Produktbeschreibung

Weitere Bezeichnung: Aconitase

Beschreibung: Aconitase (aconitate hydratase, EC 4.2.1.3) is an iron-sulfur protein containing an [Fe 4 S 4 ] 2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λ max at 450nm.

Anwendungen

Beschränkungen: Nur für Forschungszwecke einsetzbar