Adiponectin (rat) Elisa Assay Kit

Details zu Produkt Nr. ABIN411776, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACDC
  • ACRP30
  • ADIPQTL1
  • ADPN
  • APM-1
  • APM1
  • GBP28
  • apM-1
  • 30kDa
  • APN
  • Acdc
  • Acrp30
  • Ad
  • adipo
  • apM1
  • adiponectin, C1Q and collagen domain containing
  • ADIPOQ
  • Adipoq
Reaktivität
Ratte (Rattus)
56
41
27
10
9
8
8
7
5
3
2
1
1
1
1
Applikation
Biochemical Assay (BCA)
Optionen
Hersteller
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Proben Biological Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Samples
Nachweismethode Colorimetric
Produktmerkmale Rat Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in rat Serum, Plasma or Various Tissue or Cell Culture supernatants.
Plate is coated with a polyclonal antibody specific for adiponectin. Detection antibody is a monoclonal antibody specific for adiponectin.
Bestandteile Antibody coated 96-well plate (12x8 well strips)
10X Wash concentrate, 50 ml
5X Diluent, 50 ml
Secondary antibody, 12 ml
100X Detector (HRP conjugated mouse IgG), 150 μl
Standard (recombinant rat adiponectin from HEK 293cells), 1 vial, lyophilized (48 ng)
QC sample= a positive control of rat serum adiponectin, 1 vial, lyophilized (12.67 μg)
Substrate I, 6 ml
Substrate II, 6 ml
Stop solution, 12 ml
Andere Bezeichnung Adiponectin (ADIPOQ ELISA Kit Abstract)
Hintergrund Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Rat Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in rat serum, plasma or various tissue or cell culture supernatants. In the assay, polyclonal antibody specific for rat adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and any adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-rat adiponectin monoclonal antibody. With adding HRP conjugated mouse IgG and HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Forschungsgebiet Cancer, Atherosclerosis, Metabolism, Hormones
Applikationshinweise This rat Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in rat serum, plasma or various tissue or cell culture supernatants
Kommentare

Further details regarding sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Plattentyp Strips (12 x 8)
Protokoll A. Preparation of Reagents 1. Allow all samples and kit components to equilibrate to room temperature (20-25 °C).
2. Plan the plate configuration and create a plate map. Calculate the amount of working reagents to use (See table below). It is recommended that standards and samples be run in duplicate.
3. Prepare 1X Wash Solution: Dilute 5X Wash Concentrate to 1X with deionized water. The diluted 1X Wash Solution is stable for one month at room temprature
4. Prepare 1X Diluent. Dilute 5X Diluent to 1X with deionized water.
5. Prepare 1X Detector. Dilute 100X Detector to 1X wth 1X Diluent. Use the 1X Detector within one hour of preparation.
6. Prepare Substrate Solution freshly by adding one part Substrate I to one part Substrate II. Freshly prepare just before use. The amount of working reagents needed for 1 well Working reagents Total volume needed Stock solution added Dilution solution added Note 1X Wash Solution 2.8 mL 0.56 mL of 5X Wash Concentrate 2.24 mL of ddH2O Stable for 1 month at RT 1X Diluent 2 mL 0.4 mL of 5X Diluent 1.6 mL of ddH2O in the case of 10 µL sample, Including standard dilution 1X Detector 110 µL 1.1 µL of 100X Detector 108.9 µL of 1X Diluent Use within 1 hr. Substrate Solution 110 µL 55 µL of Substrate I 55 µL of Substrate II Freshly prepared just before use
7. Prepare working aliquots of the Standard as follows: When opening the lyophilized satndard, remove cap gently as the lyophilizate may have become dislodged during shipping. Add 1 mL of deionized water to the Standard vial to make a stock concentration of 48 ng/mL. Mix well. A recommended dilution scheme is as follows : 1) Label 8 microcentrifuge tubes #0-7. Add 300 µL 1X Diluent to the microcentrifuge tubes #0-7. 2) Add 300 µL of the stock Standard solution to tube #7 and vortex. This is Standard tube #7 with a concentration of 24 ng/mL 3) Standards #6 to #1 are then prepared by performing a 1:2 dilution of the preceding standard. Do not add any standard to the tube #0 300 µL 300 µL 300 µL 300 µL 300 µL 300 µL 300 µL stock Standard Tube # stock 7 6 5 4 3 2 1 0 Conc.(ng/mL) 48 24 12 6 3 1.5 0.75 0.375 0 Initial vol.(µL) - 300 300 300 300 300 300 300 300
8. Reconstitute QC sample in 1 mL of deionized water. The Kit Rev. 02/07
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Adiponectin (rat) Elisa Assay Kit (ABIN411776) Adiponectin (rat) Elisa Assay Kit
Produkt verwendet in: Cammisotto, Londono, Gingras, Bendayan: "Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats." in: American journal of physiology. Renal physiology, Vol. 294, Issue 4, pp. F881-9, 2008 (PubMed).

Sevillano, de Castro, Bocos, Herrera, Ramos: "Role of insulin receptor substrate-1 serine 307 phosphorylation and adiponectin in adipose tissue insulin resistance in late pregnancy." in: Endocrinology, Vol. 148, Issue 12, pp. 5933-42, 2007 (PubMed).

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