MMP2 ELISA Kit

Produktnummer ABIN411386

Produktdetails MMP2 ELISA Kit

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Bindungsspezifität: AA 30-662

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625-40000 pg/mL

Untere Nachweisgrenze: 625 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MMP-2

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A30-C662

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A30-C662

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase M10A family.

Plattentyp: Pre-coated

Protokoll: rat MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-2 has been precoated onto 96-well plates. Standards(NSO, A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 40000pg/mL, 20,000pg/mL,10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MMP-2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1358, Standard deviation: 59.8, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 3628, Standard deviation: 206.8, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 6327, Standard deviation: 399, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 1526, Standard deviation: 82.4, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 3874, Standard deviation: 240.2, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 6556, Standard deviation: 465.5, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für MMP2 ELISA Kit (ABIN411386)

You, Zu, Liu, Kong, Song, Wei, Zhou, Wang, Yan: "Synovial fibroblast-targeting liposomes encapsulating an NF-κB-blocking peptide ameliorates zymosan-induced synovial inflammation." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2449-2457, (2018) (PubMed).

Zhang, Shi, Zou, Chen, Tang, Ye, Liu: "KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 135-140, (2017) (PubMed).

Zhang, Li, Liu, Wang, Chen, Xing, Zhu: "STAT3-mediated MMP-2 expression is required for 15-HETE-induced vascular adventitial fibroblast migration." in: The Journal of steroid biochemistry and molecular biology, Vol. 149, pp. 106-17, (2015) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." in: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

Qin, Liu, Li, Liu, Li, Gao, Shao, Zhen: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." in: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).

Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." in: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." in: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

Zhang, Sun, Zhang, Ge, Liu, Zhao, Lu, Fan: "Norcantharidin inhibits tumor growth and vasculogenic mimicry of human gallbladder carcinomas by suppression of the PI3-K/MMPs/Ln-5?2 signaling pathway." in: BMC cancer, Vol. 14, pp. 193, (2014) (PubMed).

Rizk, El-Maraghy, Nassar: "A novel role for SIRT-1 in L-arginine protection against STZ induced myocardial fibrosis in rats." in: PLoS ONE, Vol. 9, Issue 12, pp. e114560, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." in: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

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Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: MMP2 (MMP2 Produkte)

Synonyme: 2-MMP ELISA Kit, CG1794 ELISA Kit, DM2-MMP ELISA Kit, Dm2-MMP ELISA Kit, Dmel\\CG1794 ELISA Kit, MMP2 ELISA Kit, anon-WO0118547.84 ELISA Kit, dm-2MMP ELISA Kit, dmmp2 ELISA Kit, l(2)02353 ELISA Kit, mmp2 ELISA Kit, wu:fa99h12 ELISA Kit, wu:fk89d01 ELISA Kit, fgmmp-2 ELISA Kit, Mmp-2 ELISA Kit, LOC100135793 ELISA Kit, Clg4a ELISA Kit, GelA ELISA Kit, MMP-2 ELISA Kit, CLG4 ELISA Kit, CLG4A ELISA Kit, MMP-II ELISA Kit, MONA ELISA Kit, TBE-1 ELISA Kit, matrix metallopeptidase 2 ELISA Kit, Matrix metalloproteinase 2 ELISA Kit, matrix metalloproteinase 2 ELISA Kit, matrix metalloproteinase-2 ELISA Kit, MMP2 ELISA Kit, Mmp2 ELISA Kit, mmp2 ELISA Kit, LOC657982 ELISA Kit, LOC100135793 ELISA Kit

Hintergrund:

Protein Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity). .

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Synonyms: 72 kDa type IV collagenase,3.4.24.24,72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2,PEX,Mmp2,

Full Gene Name: 72 kDa type IV collagenase

Cellular Localisation: Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity)..

Gen-ID: 81686

UniProt: P33436

Pathways: Activation of Innate immune Response