Growth Hormone 1 ELISA Kit

Produktnummer ABIN411378

Produktdetails Growth Hormone 1 ELISA Kit

Target: Growth Hormone 1 (GH1)

Bindungsspezifität: AA 27-217

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Growth Hormone

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: F27-F217

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: F27-F217

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli, F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human GH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human GH standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 101, Standard deviation: 4.44, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 12.06, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 618, Standard deviation: 24.10, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 9.922, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 26.55, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 596, Standard deviation: 43.51, CV(%): 7.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Andere Bezeichnung: GH1 (GH1 Produkte)

Synonyme: GH ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, IGHD1B ELISA Kit, hGH-N ELISA Kit, Gh1 ELISA Kit, GH1 ELISA Kit, gh ELISA Kit, Gh ELISA Kit, GH2 ELISA Kit, RNGHGP ELISA Kit, ghl ELISA Kit, gh-n ELISA Kit, ghn ELISA Kit, ighd1b ELISA Kit, gh1 ELISA Kit, ghb-A ELISA Kit, GHI ELISA Kit, GHB3 ELISA Kit, growth hormone 1 ELISA Kit, growth hormone ELISA Kit, somatotropin-like ELISA Kit, somatotropin ELISA Kit, growth hormone prepeptide ELISA Kit, growth hormone 1 L homeolog ELISA Kit, growth hormone 1 S homeolog ELISA Kit, Somatotropin-1 ELISA Kit, GH1 ELISA Kit, Gh ELISA Kit, GH ELISA Kit, LOC100305005 ELISA Kit, Gh1 ELISA Kit, LOC100534452 ELISA Kit, LOC100232594 ELISA Kit, LOC100303681 ELISA Kit, gh1 ELISA Kit, LOC100356068 ELISA Kit, LOC100136588 ELISA Kit, gh1.L ELISA Kit, gh1.S ELISA Kit, LOC109081196 ELISA Kit

Hintergrund:

Background: Growth hormone(GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone(GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency(GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.

Synonyms: Growth hormone 1 isoform 1 ,HCG1749481, isoform CRA_s ,GH1 ,hCG_1749481 ,

Full Gene Name: growth hormone 1

Cellular Localisation: Secreted.

Gen-ID: 2688

UniProt: B1A4G6

Pathways: NF-kappaB Signalweg, JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process