Thrombopoietin ELISA Kit

Produktnummer ABIN411356

Produktdetails Thrombopoietin ELISA Kit

Target: Thrombopoietin (THPO)

Bindungsspezifität: AA 22-356

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TPO

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S22-T356

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S22-T356

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Found mainly in the liver, kidney and skeletal muscle.

Plattentyp: Pre-coated

Protokoll: mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards(NSO, S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TPO amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 296, Standard deviation: 11.84, CV(%): 4
• Sample 2: n=16, Mean(pg/ml): 611, Standard deviation: 33, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 1265, Standard deviation: 82.23, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 321, Standard deviation: 16.7, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 746, Standard deviation: 47, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 1464, Standard deviation: 105.4, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Thrombopoietin ELISA Kit (ABIN411356)

Wu, Chen: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin (TPO) (THPO Produkte)

Synonyme: MGDF ELISA Kit, MKCSF ELISA Kit, ML ELISA Kit, MPLLG ELISA Kit, THCYT1 ELISA Kit, TPO ELISA Kit, Mgdf ELISA Kit, Ml ELISA Kit, Mpllg ELISA Kit, Tpo ELISA Kit, Tpo1 ELISA Kit, Tpo2 ELISA Kit, Tpo3 ELISA Kit, Tpo4 ELISA Kit, tpo ELISA Kit, LOC100231762 ELISA Kit, thrombopoietin ELISA Kit, THPO ELISA Kit, Thpo ELISA Kit, thpo ELISA Kit

Hintergrund:

Protein Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Background: Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Synonyms: Thrombopoietin,C-mpl ligand,ML,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,MGDF,Myeloproliferative leukemia virus oncogene ligand,Thpo,

Full Gene Name: Thrombopoietin

Cellular Localisation: Secreted.

Gen-ID: 21832

UniProt: P40226

Pathways: JAK-STAT Signalweg, Hormone Activity