MMP1 ELISA Kit

Produktnummer ABIN411327

Produktdetails MMP1 ELISA Kit

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Bindungsspezifität: AA 20-469

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: F20-N469

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <20pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: F20-N469

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human MMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-1 has been precoated onto 96-well plates. Standards(NSO, F20-N469) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 937, Standard deviation: 42.2, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 3127, Standard deviation: 159.5, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 5438, Standard deviation: 293.7, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 1259, Standard deviation: 74.3, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 3456, Standard deviation: 207.4, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 5862, Standard deviation: 393, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für MMP1 ELISA Kit (ABIN411327)

Lei, Zeng, Lu, Ding, Xia, Kang, Tan, Gao, Kang, Cao, Zhou, Xiao, Chen, Huang: "MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 3977-3982, (2018) (PubMed).

Zeng, Zhou, Lei, Chen, Lu, Zhou, Cao, Gao, Xia, Ding, Huang, Xiang, Wang, Xiao, Xiao, Huang: "Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging." in: Molecular medicine reports, Vol. 15, Issue 1, pp. 111-116, (2017) (PubMed).

Nosratzehi, Alijani, Moodi: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Deng, Ling, Liu, Zhao, Lv: "Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 992-998, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Nikniaz, Ostadrahimi, Mahdavi, Ebrahimi, Nikniaz: "Effects of Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis." in: Complementary therapies in medicine, Vol. 22, Issue 5, pp. 864-9, (2014) (PubMed).

Ågren, Schnabel, Christensen, Mirastschijski: "Tumor necrosis factor-?-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo." in: European journal of cell biology, Vol. 94, Issue 1, pp. 12-21, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." in: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

An, Wu, Lin: "Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts." in: International journal of ophthalmology, Vol. 4, Issue 4, pp. 353-6, (2012) (PubMed).

Huang, Luo, Lu, Chen, Zuo, Xiang, Yang, Tan, Kang, Bi: "IPL irradiation rejuvenates skin collagen via the bidirectional regulation of MMP-1 and TGF-?1 mediated by MAPKs in fibroblasts." in: Lasers in medical science, Vol. 26, Issue 3, pp. 381-7, (2011) (PubMed).

Details zu MMP1

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: MMP1 (MMP1 Produkte)

Synonyme: CLG ELISA Kit, CLGN ELISA Kit, Mmp1a ELISA Kit, CG4859 ELISA Kit, Dm1-MMP ELISA Kit, Dmel\\CG4859 ELISA Kit, MMP-1 ELISA Kit, MMP1 ELISA Kit, Mmp 1 ELISA Kit, dMMP1 ELISA Kit, dm1-MMP ELISA Kit, dmmp1 ELISA Kit, l(2)k04809 ELISA Kit, mmp1 ELISA Kit, Mmp1 ELISA Kit, Mcol-A ELISA Kit, Mcola ELISA Kit, col4 ELISA Kit, mmp18 ELISA Kit, Clgn ELISA Kit, matrix metallopeptidase 1 ELISA Kit, Matrix metalloproteinase 1 ELISA Kit, matrix metalloproteinase 1 ELISA Kit, matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1a (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1 S homeolog ELISA Kit, interstitial collagenase ELISA Kit, matrix metallopeptidase 8 L homeolog ELISA Kit, MMP1 ELISA Kit, Mmp1 ELISA Kit, RB11133 ELISA Kit, Mmp1a ELISA Kit, mmp1.S ELISA Kit, LOC100727966 ELISA Kit, mmp8.L ELISA Kit

Hintergrund:

Background: Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

Synonyms: cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7) ,

Full Gene Name: matrix metallopeptidase 1 (interstitial collagenase)

Gen-ID: 4312

UniProt: B4DN15