IL12A ELISA Kit (Interleukin 12 alpha)

Details for Product IL12A ELISA Kit No. ABIN411309, Anbieter: Anmelden zum Anzeigen
Antigen
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • Il12a
  • IL12A
Alternativen
Cow (Bovine) IL12A ELISA Kit
Epitop
AA 23-328, AA 23-219
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
12
8
7
6
5
5
3
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
7.8-500 pg/mL
Untere Nachweisgrenze
7.8 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-12(p70)
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: sf21
Immunogen sequence: (I23-S328)+(R23-S219)
Spezifität Expression system for standard: sf21
Immunogen sequence: (I23-S328)+(R23-S219)
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <2pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Andere Bezeichnung IL12A (IL12A ELISA Kit Abstract)
Hintergrund

Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Synonyms: Interleukin 12 ,Interleukin 12, P35 ,Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ,Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ,IL12A ,hCG_1685939 ,

Full Gene Name: interleukin 12A

Gen-ID 3592
UniProt O60595
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll human IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-12(P70) has been precoated onto 96-well plates. Standards(sf21, (I23-S328)+(R23-S219)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-12(P70) amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-12(P70) standard solution and each sample is measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 44, Standard deviation: 2.16, CV(%): 4.9
  • Sample 2: n=16, Mean(pg/ml): 127, Standard deviation: 7.24, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 283, Standard deviation: 17.83, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 67, Standard deviation: 3.89, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 158, Standard deviation: 9.48, CV(%): 6
  • Sample 3: n=24, Mean(pg/ml): 322, Standard deviation: 23.2, CV(%): 7.2
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Interleukin 12 alpha (IL12A) ELISA Kit (ABIN411309) Human IL-12(p70) PicoKine ELISA Kit standard curve
Produkt verwendet in: Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014 (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014 (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014 (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014 (PubMed).

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013 (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012 (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012 (PubMed).

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011 (PubMed).

Li, Yu, Ye, Wang, Yang: "Lactobacillus S-layer protein inhibition of Salmonella-induced reorganization of the cytoskeleton and activation of MAPK signalling pathways in Caco-2 cells." in: Microbiology (Reading, England), Vol. 157, Issue Pt 9, pp. 2639-46, 2011 (PubMed).

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