EggsPure IgY (6 eggs) antibody purification kit

Applikation: Immunpräzipitation (IP)

Produktnummer: ABIN410201

Produktbeschreibung

Produktmerkmale: The EggsPure IgY kit provides a quick, simple and efficient method to extract IgY from egg yolk. Using EggsPure IgY, total IgY yields around 50 mg with a purity of at least 90 %. IgY thus purified are suitable for direct use in ELISA, immunoblotting, flow cytometry, immunochromatography and affinity purification methods. After poultry immunization, specific IgY antibodies can be then purified, from the total IgY extracted by the EggsPure IgY, by affinity chromatography using specific antigen immobilized in a chromatography column. Specific IgY antibodies represent 2 to 10 % of total IgY antibodies.

Spezifität: IgY purification from egg yolk. This kit also works with duck, goose and quail eggs.

Anwendungen

Protokoll: A protocol is provided with the product. This protocole is available in english, spanish or french.

Bestandteile: Reagent A: 420 mL, Reagent B: 380 mL, Reagent C: 265 mL, Agro-Bio egg separator, 6 fast-flow filter paper, 2 plastic hooks, Record sheet (in english, spanish or french)

Benötigtes Material: Phosphate Buffered Saline (PBS 1X), Centrifuge and centrifuge tubes, Funnel, Magnetic stir bar and magnetic stirrer, Spatula, Graduated cylinders

Lagerung: The EggsPure kit is stable 24 months at 2-8°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar