Ovalbumin (OVA) ELISA Kit

Antigen: Ovalbumin (OVA)

Synonyme: LOC396058, R86, Spi10, SPI-CI, ovalbumin, 1600019A21Rik, RP23-391I11.4, NK13, Spi12, NK21, Spi13, NK21L1, BC052216, MGC61007, NK26, Spi14, AW540195, Spi3, AI876477, Serpinb6, 4930482L21Rik, D330015H01Rik, PI9, CAP3, PI-9, Spi6, CAP-3, BB283241, Spi7, Maspin, AI462524, AI646751, 1110036M19Rik, CAP2, NK10, Spi8, CAP-2, AT2, Spi9, SPIC, SPI3C, C76171, C76174, RP23-262J21.6, megsin, 4631416M05Rik, EIB, Serpin1b1, 6330533H24Rik, EID, EIC, MGC129308, SPI3B, Gm11396, OTTMUSG00000000720

Reaktivität: Huhn

Applikation: ELISA

Produktnummer: ABIN410194

Produktbeschreibung

Produktmerkmale: Enzyme-Linked Immuno Sorbent Assay (ELISA) kit for detection and quantification of soluble ovalbumin

Weitere Bezeichnung: Ovalbumin

Spezifität: Detection of soluble native ovalbumin

Sensitivität: Limit of detection: 2 ng/mL

Synonyme: LOC396058, R86, Spi10, SPI-CI, ovalbumin, 1600019A21Rik, RP23-391I11.4, NK13, Spi12, NK21, Spi13, NK21L1, BC052216, MGC61007, NK26, Spi14, AW540195, Spi3, AI876477, Serpinb6, 4930482L21Rik, D330015H01Rik, PI9, CAP3, PI-9, Spi6, CAP-3, BB283241, Spi7, Maspin, AI462524, AI646751, 1110036M19Rik, CAP2, NK10, Spi8, CAP-2, AT2, Spi9, SPIC, SPI3C, C76171, C76174, RP23-262J21.6, megsin, 4631416M05Rik, EIB, Serpin1b1, 6330533H24Rik, EID, EIC, MGC129308, SPI3B, Gm11396, OTTMUSG00000000720

Anwendungen

Prinzip: It is an immunoenzymatic one-step assay based on polyclonal antibodies against ovalbumin, one immobilized on the microwells, and the other conjugated to horseradish peroxidase (HRP). Samples and anti-ovalbumin HRP conjugate are dispensed into the wells and incubated simultaneously at room temperature (20-25°C). After a washing step which eliminates unbound components, HRP substrate (TMB) is added to each well and color (blue) is developed by the enzymatic reaction of HRP on the substrate, which is directly proportional to the amount of ovalbumin present in the sample. Reaction stops by addition of sulphuric acid to the wells, these results in a colour change to yellow. Absorbance can thus be measured at 450 nm and at 620 nm for the reference. The concentration of ovalbumin in samples and control is calculated from a standard curve of standards.

Protokoll: A protocol is provided with the product. This protocole is available in english or french.

Bestandteile: 1 plastic strips holder. 4 zip lock bags containing each: 3 8-well strips coated with polyclonal antibodies (reagent 1), 1 stock solution of ovalbumin (S4 solution) (reagent 2), 1 positive control (reagent 3), 1 HRP-labelled polyclonal anti-ovalbumin antibodies, 1 solution of tetramethylbenzidine (TMB), HRP substrate (reagent 5), 1 sample buffer (reagent 6), 1 wash buffer (reagent 7)

Benötigtes Material: Tubes, Sulfuric acid 1 N (0.5 mol/L), Microplate reader with 450 and 620 nm filters, Usual laboratories equipment (Vortex mixer, parafilm, chronometer, multichannel pipette, stock bottle, distilled water,

Lagerung: This kit is stable 12 months at 2-8°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar