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PEDF ELISA Kit

SERPINF1 Reaktivität: Ratte Colorimetric Sandwich ELISA 6.25-400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN367530
  • Target Alle PEDF (SERPINF1) ELISA Kits anzeigen
    PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
    Reaktivität
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    6.25-400 pg/mL
    Untere Nachweisgrenze
    6.25 pg/mL
    Applikation
    ELISA
    Proben
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
    Analytische Methode
    Quantitative
    Kreuzreaktivität (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivität
    1.56 pg/mL
    Bestandteile
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
    Featured
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    Discover our top product SERPINF1 ELISA Kit
  • Applikationshinweise
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Kommentare

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Testpräzision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handhabung
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Haltbarkeit
    6 months
  • Zhang, Pan, Qiu, Wei, Li, Jiang, Zhuang, Liu, Zhang, Dong: "A novel modified physiologically relevant model for cardiac angiogenesis." in: Microvascular research, Vol. 114, pp. 84-91, (2018) (PubMed).

    Zhang, Wei, Jiang, Li, Cui, Pan, Zhuang, Sun, Liu, Zhang, Dong: "PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-γ to increase surface FasL." in: Apoptosis : an international journal on programmed cell death, Vol. 21, Issue 1, pp. 60-8, (2016) (PubMed).

    Şahin, Apaydın, Töz, Sivrikoz, Genç, Turan, Cengiz, Eskicioğlu et al.: "Comparison of the effects of letrozole and cabergoline on vascular permeability, ovarian diameter, ovarian tissue VEGF levels, and blood PEDF levels, in a rat model of ovarian hyperstimulation ..." in: Archives of gynecology and obstetrics, Vol. 293, Issue 5, pp. 1101-6, (2016) (PubMed).

    Zhuang, Zhang, Pan, Li, Wei, Cui, Liu, Guan, Dong, Zhang: "PEDF and PEDF-derived peptide 44mer inhibit oxygen-glucose deprivation-induced oxidative stress through upregulating PPARγ via PEDF-R in H9c2 cells." in: Biochemical and biophysical research communications, Vol. 472, Issue 3, pp. 482-8, (2016) (PubMed).

    Turan, Eskicioglu, Sivrikoz, Cengiz, Gur, Tatar, Sahin, Yilmaz: "Prophylactic vitamin D supplementation in ovarian hyperstimulation syndrome: an animal study." in: Archives of gynecology and obstetrics, (2015) (PubMed).

    Turan, Eskicioglu, Sivrikoz, Cengiz, Adakan, Gur, Tatar, Sahin, Yilmaz: "Myo-inositol is a promising treatment for the prevention of ovarian hyperstimulation syndrome (OHSS): an animal study." in: Archives of gynecology and obstetrics, (2015) (PubMed).

    Zhang, Wang, Feng, Xu, Shi, Chen, Yuan, Yan, Zhuang, Zhang, Zhang, Dong: "PEDF improves cardiac function in rats with acute myocardial infarction via inhibiting vascular permeability and cardiomyocyte apoptosis." in: International journal of molecular sciences, Vol. 16, Issue 3, pp. 5618-34, (2015) (PubMed).

    Vasanth, Arul, Karthikeyeni, Kumar, Vignesh, Manimegalai, Bupesh, Thirumurugan, Subramanian: "Influence of Triazine Herbicide Exposure on Guppies (Poecilia sphenops) Aromatase Activities, Altered Sex Steroid Concentration and Vitellogenin Induction." in: Indian journal of pharmaceutical sciences, Vol. 77, Issue 2, pp. 156-62, (2015) (PubMed).

    Ozdemir, K?l?nç, Ergün, Sahin: "Rapamycin inhibits oxidative and angiogenic mediators in diabetic retinopathy." in: Canadian journal of ophthalmology. Journal canadien d'ophtalmologie, Vol. 49, Issue 5, pp. 443-9, (2014) (PubMed).

    Gao, Zhang, Zhuang, Yuan, Sun, Jiang, Zhou, Yuan, Zhang, Dong: "PEDF and PEDF-derived peptide 44mer protect cardiomyocytes against hypoxia-induced apoptosis and necroptosis via anti-oxidative effect." in: Scientific reports, Vol. 4, pp. 5637, (2014) (PubMed).

  • Target Alle PEDF (SERPINF1) ELISA Kits anzeigen
    PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
    Andere Bezeichnung
    Pigment epithelium-derived factor (PEDF) (SERPINF1 Produkte)
    Synonyme
    EPC-1 ELISA Kit, OI12 ELISA Kit, OI6 ELISA Kit, PEDF ELISA Kit, MGC82984 ELISA Kit, SERPINF1 ELISA Kit, AI195227 ELISA Kit, Pedf ELISA Kit, Pedfl ELISA Kit, Sdf3 ELISA Kit, Dmrs91 ELISA Kit, zgc:92072 ELISA Kit, serpin family F member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 1 ELISA Kit, SERPINF1 ELISA Kit, serpinf1 ELISA Kit, serpinf1.L ELISA Kit, Serpinf1 ELISA Kit
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